Identification and Quantitation of Reaction Intermediates and Residuals in Lipase-Catalyzed Transesterified Oils by HPLC

A high-performance liquid chromatography (HPLC) unit equipped with size exclusion column and a refractive index detector was used for simultaneous monitoring, identification, and quantitation of the reaction components from lipase-catalyzed transesterification of three oils. The procedure simultaneo...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2011-09, Vol.165 (1), p.155-177
Main Authors: Aryee, Alberta N. A, Phillip, Leroy E, Cue, Roger I, Simpson, Benjamin K
Format: Article
Language:English
Subjects:
alcohols
Animals
Biochemistry
Biological and medical sciences
Biotechnology
Catalysis
Chemistry
Chemistry and Materials Science
Chromatography, High Pressure Liquid - methods
coproducts
derivatization
Diglycerides - analysis
Enzymes
Fish Oils - chemistry
free fatty acids
Fundamental and applied biological sciences. Psychology
high performance liquid chromatography
Lipase - metabolism
Liquid chromatography
Monoglycerides - analysis
Oils & fats
Olive Oil
Plant Oils - chemistry
quantitative analysis
refractive index
Salmon
salmon oil
transesterification
triacylglycerol lipase
triacylglycerols
Triglycerides - analysis
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Summary:A high-performance liquid chromatography (HPLC) unit equipped with size exclusion column and a refractive index detector was used for simultaneous monitoring, identification, and quantitation of the reaction components from lipase-catalyzed transesterification of three oils. The procedure simultaneously separated and detected the unreacted triacylglycerols (TAG), diacyl-, and monoacyl-glycerol (DAG and MAG) co-products, residual alcohol as well as free fatty acid (FFA) based on retention times. The chromatograms showed well separated and resolved peaks. The elution of the components from the transesterification reaction in increasing order was: TAG 
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-011-9241-z