Comparison of time to positivity of the VersaTREK® REDOX 80-mL and the REDOX EZ draw 40-mL blood culture bottles for common bacterial bloodstream pathogens

Abstract The VersaTREK® microbial detection system offers 2 media formulations, an aerobic and an anaerobic bottle available in a 40-mL direct draw format and an 80-mL format. The 40-mL EZ Draw® bottle can be inoculated with a maximum volume of 5 mL, while the REDOX 80-mL bottle accommodates a 10-mL...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2011-10, Vol.71 (2), p.101-105
Main Authors: Samuel, Linoj P, Pimentel, Jason D, Tibbetts, Robert J, Martin, Rebekah, Hensley, Rhonda, Meier, Frederick A
Format: Article
Language:English
Subjects:
Aerobiosis
Anaerobiosis
Bacteremia - diagnosis
Bacteremia - microbiology
Bacteria - growth & development
Bacteria - isolation & purification
Bacteriological Techniques
Bacteriology
Biological and medical sciences
Blood - microbiology
Blood cultures
Culture Media
Fundamental and applied biological sciences. Psychology
Humans
Infectious Disease
Infectious diseases
Internal Medicine
Medical sciences
Microbiology
Miscellaneous
Reagent Kits, Diagnostic
Time to positivity
VersaTREK
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract The VersaTREK® microbial detection system offers 2 media formulations, an aerobic and an anaerobic bottle available in a 40-mL direct draw format and an 80-mL format. The 40-mL EZ Draw® bottle can be inoculated with a maximum volume of 5 mL, while the REDOX 80-mL bottle accommodates a 10-mL volume. The effect of volume of blood inoculum on time to positivity (TTP) has not been clearly established with these bottle types. This study utilized simulated blood cultures seeded with clinically relevant microorganisms in human blood to evaluate the impact of inoculum volume and organism load on TTP for the 2 bottle types. For 13/15 organisms, the EZ Draw bottle flagged positive earlier than the REDOX 80-mL bottles. The lower volume of blood inoculum did not negatively impact TTP using the EZ Draw blood culture bottles as compared to REDOX 80-mL bottles.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2011.06.001