Callus culture of Echium italicum L. towards production of a shikonin derivative

Callus induction and proliferation of Echium italicum L. (Boraginaceae) were investigated using cotyledon, hypocotyl and root explants. Calli were initiated and established using B5, LS, 1/2LS and White media supplemented with different auxins, including 2,4-dichlorophenoxyacetic acid (2,4-D), indol...

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Bibliographic Details
Published in:Natural product research 2011-09, Vol.25 (16), p.1480-1487
Main Authors: Zare, Kh, Khosrowshahli, M., Nazemiyeh, H., Movafeghi, A., Azar, A. Motallebi, Omidi, Y.
Format: Article
Language:English
Subjects:
Anthraquinones - chemistry
Anthraquinones - isolation & purification
Boraginaceae
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Cotyledon - chemistry
Culture Media - chemistry
Echium
Echium - chemistry
Echium italicum
Hypocotyl - chemistry
Indoleacetic Acids - chemistry
Kinetin - chemistry
Mass Spectrometry
pigmented callus
Plant Roots - chemistry
preparative HPLC
shikonin acetate
Spectrophotometry, Ultraviolet
tissue culture
Tissue Culture Techniques - methods
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Summary:Callus induction and proliferation of Echium italicum L. (Boraginaceae) were investigated using cotyledon, hypocotyl and root explants. Calli were initiated and established using B5, LS, 1/2LS and White media supplemented with different auxins, including 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA) and 1-naphthaleneacetic acid (NAA) in combination with kinetin. The maximum pigmented callus induction (100%) was observed in the White medium. The n-hexane extract of proliferated callus tissues were analysed by TLC and HPLC. The major secondary metabolite was separated by preparative HPLC and its structure was elucidated by UV, 1 H and 13 C-NMR spectroscopy. As a result, shikonin acetate was identified by various spectroscopic methods from callus culture of E. italicum. These findings highlight the shikonin production potential of the E. italicum callus, which may be considered as a new source for the production of shikonin and its derivatives for industrial use.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786410902804857