Analysis of peptidyl-propyl-cis/trans isomerase 1 (PIN1) gene −842(G > C) and −667(T > C) polymorphic variants in relation to breast cancer risk and clinico-pathological parameters

• Published in: Scandinavian journal of clinical and laboratory investigation 2011-10, Vol.71 (6), p.500-506
• Main Authors: Naidu, Rakesh, Har, Yip C., Taib, Nur A. M.
• Format: Article
• Language: English
• Subjects: Adult; Amplified Fragment Length Polymorphism Analysis; Biological and medical sciences; Breast cancer; Breast Neoplasms - ethnology; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Case-Control Studies; China - ethnology; Female; Gene Frequency; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; genotyping; Gynecology. Andrology. Obstetrics; Humans; India - ethnology; Investigative techniques, diagnostic techniques (general aspects); Lymphatic Metastasis; Malaysia; Mammary gland diseases; Medical sciences; Middle Aged; Neoplasm Grading; Neoplasm Staging; NIMA-Interacting Peptidylprolyl Isomerase; PCR-RFLP; Peptidylprolyl Isomerase - genetics; PIN1; polymorphism; Polymorphism, Single Nucleotide; Receptors, Estrogen - metabolism; Receptors, Progesterone - metabolism; Risk Factors; Tumors
• ISSN: 0036-5513; 1502-7686
• DOI: 10.3109/00365513.2011.590223

Abstract Background. The purpose of this study was to investigate the association between the peptidyl-propyl-cis/trans isomerase 1 (PIN1) −842(G > C) and −667(T > C) polymorphic variants and breast cancer risk among Malaysian ethnic groups namely the Malays, Chinese and Indians, as well as clinico-pathological characteristics of the patients. Patients and Methods. The polymerase chain reaction-restriction fragment length polymorphism was used to genotype 387 breast cancer patients and 252 normal and healthy women who had no history of any malignancy. Results. The distribution of −842(G > C) and −667(T > C) genotypes and alleles frequencies between breast cancer cases and normal individuals showed lack of statistical significance among the Malays (p > 0.05), Chinese (p > 0.05) and Indians (p > 0.05), respectively. Multivariate logistic regression analysis showed that the Malay, Chinese and Indian women who were −842CC homozygotes (p = 0.198, 0.089, 0.620), −842GC heterozygotes (p = 0.492, 0.176, 0.377) and −842C allele carriers (P = 0.226, 0.059, 0.669), respectively, were not associated with breast cancer risk. Furthermore Malay, Chinese and Indian women who were heterozygous (p = 0.777, 0.319, 0.710) and homozygous (p = 0.864, 0.986, 0.954) for −667C allele or carriers of −667C allele (p = 0.977, 0.915, 0.880), respectively, were not associated with an increased risk of breast cancer. None of the −842C and −667C allele genotypes were significantly associated with the clinico-pathological characteristics. Conclusion. Our findings suggest that the polymorphic variants of −842(G > C) and −667(T > C) genes may not appear to have an influence on breast cancer risk among Malaysian Malay, Chinese and Indian women.