Comparison of genetic variability between Czech and foreign isolates of phytopathogenic bacteriaClavibacter michiganensis subsp.sepedonicus by Rep-PCR technique

Repetitive-sequence-based polymerase chain reaction (Rep-PCR) method was used for analysis of genetic variability among bacterial populations from different world locations. Collection of 26 Czech and 13 foreign strains ofClavibacter michiganensis subsp.sepedonicus was amplified using BOX primer tar...

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Bibliographic Details
Published in:Folia microbiologica 2002-08, Vol.47 (4), p.450-454
Main Authors: Fousek, J., Mráz, I., Petrzik, K.
Format: Article
Language:English
Subjects:
Bacteria
Cluster analysis
Genetic analysis
Genetic variability
Genetics
Genomes
Polymerase chain reaction
Population genetics
Populations
Primers
Strains (organisms)
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Summary:Repetitive-sequence-based polymerase chain reaction (Rep-PCR) method was used for analysis of genetic variability among bacterial populations from different world locations. Collection of 26 Czech and 13 foreign strains ofClavibacter michiganensis subsp.sepedonicus was amplified using BOX primer targeting to repetitive motif occurring in eubacterial genomes. Genetic fingerprints were visually compared and statistically evaluated by cluster analysis. Genetic similarity was estimated to be approximately 80% among all tested strains. Populations of these bacteria seem to be highly homogeneous; potential influence of geographic origin was not confirmed.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02818707