Synergisitic Effect of Chitinases and Bacillus Thuringiensis israelensis Spore-Toxin Complex Against Aedes aegypti Larvae

Six subspecies of Bacillus thuringiensis Berliner (Bt) were grown in minimal medium with chitin as the sole carbon source for 6 days to obtain Bt cell-free fermented broths, which were then evaluated for chitinolytic activity and tested against third-instar Aedes aegypti (L.) (Diptera: Culicidae) la...

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Bibliographic Details
Published in:Canadian entomologist 2011-04, Vol.143 (2), p.157-164
Main Authors: Ramírez-Suero, Montserrat, Valerio-Alfaro, Gerardo, Bernal, Julio S., Ramírez-Lepe, Mario
Format: Article
Language:English
Subjects:
Aedes aegypti
Bacillus thuringiensis
Bacillus thuringiensis israelensis
Biochemistry
Culicidae
Diptera
Entomology
Larvae
Mosquitoes
Nonnative species
Physiology, Biochemistry, Development & Genetics / Physiologie, biochimie, dé veloppement et génétique
Sciences biologiques et medicales
Sciences biologiques fondamentales et appliquees. Psychologie
Serratia marcescens
Streptomyces griseus
Toxicity
Toxins
Vecteurs d'importance medicale, nuisances, depredateurs des denrees et materiaux: surveillance des populations et lutte
Vecteurs. Hôtes intermédaires
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Summary:Six subspecies of Bacillus thuringiensis Berliner (Bt) were grown in minimal medium with chitin as the sole carbon source for 6 days to obtain Bt cell-free fermented broths, which were then evaluated for chitinolytic activity and tested against third-instar Aedes aegypti (L.) (Diptera: Culicidae) larvae. Bt Pakistani showed the highest chitinolytic activity (approximately >2700 mU/mL), Bt kurstaki showed the lowest activity (approximately
ISSN:0008-347X
1918-3240
DOI:10.4039/n10-051