Phylogenetic characterization and ochratoxin A – Fumonisin profile of black Aspergillus isolated from grapes in Argentina

Aspergillus section Nigri populations isolated from seven growing regions from Argentina were characterized by sequencing in order to identify species responsible for production of ochratoxin A (OTA) and fumonisins (FB s). Sequences of genes encoding calmodulin, β-tubulin, the second largest subunit...

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Bibliographic Details
Published in:International journal of food microbiology 2011-09, Vol.149 (2), p.171-176
Main Authors: Chiotta, M.L., Susca, A., Stea, G., Mulè, G., Perrone, G., Logrieco, A., Chulze, S.N.
Format: Article
Language:English
Subjects:
Argentina
Aspergillus
Aspergillus - classification
Aspergillus - genetics
Aspergillus - metabolism
Aspergillus section Nigri
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Fumonisins
Fumonisins - chemistry
Fumonisins - metabolism
Grapes
Ochratoxin A
Ochratoxins - chemistry
Ochratoxins - metabolism
Phylogeny
Sequencing
Tubulin - genetics
Tubulin - metabolism
Vitaceae
Vitis - microbiology
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Summary:Aspergillus section Nigri populations isolated from seven growing regions from Argentina were characterized by sequencing in order to identify species responsible for production of ochratoxin A (OTA) and fumonisins (FB s). Sequences of genes encoding calmodulin, β-tubulin, the second largest subunit of RNA polymerase II and translation elongation factor 1 alpha were analysed. The phylogenetic analysis showed the presence of six lineages: A. carbonarius, A. tubingensis, A. niger, A. japonicus, A. homomorphus and A. foetidus grouped in four major clusters. The molecular tools used allowed the identification for the first time of A. homomorphus from vineyards. OTA production confirmed the importance of A. carbonarius as the main ochratoxigenic species isolated and, to a variable degree, of A. niger and A. tubingensis, which were by far the most commonly occurring species on grapes in Argentina. The only strains able to produce OTA and fumonisins (B 2–B 4) belong to the A. niger cluster. ► Aspergillus section Nigri populations were grouped into four main clusters. ► Aspergillus tubingensis strains were isolated frequently and showed different OTA levels. ► The strains included in A. niger cluster produced both toxins, OTA and FB 2 and FB 4.
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2011.06.002