Cell therapy for fibrotic interstitial pulmonary disease: Experimental study

• Published in: Microscopy research and technique 2011-10, Vol.74 (10), p.957-962
• Main Authors: Cabral, Rosa M., BRANCO, ÉRIKA, Rizzo, Márcia Dos S., Ferreira, Guilherme J., Gregores, Guilherme B., Samoto, Vivian Y., Stopiglia, Ângelo J., Maiorka, Paulo C., Fioretto, Emerson T., Capelozzi, Vera L., Borges, João B., Gomes, Susimeire, Beraldo, Marcelo A., Carvalho, Carlos R. R., Miglino, Maria A.
• Format: Article
• Language: English
• Subjects: Animals; Bone Marrow Transplantation; cell therapy; Cell- and Tissue-Based Therapy; Disease Models, Animal; Female; Fibrosis; fibrotic interstitial pulmonary disease; histological changes in lung; Histology; Humans; Interstitials; Leukocytes, Mononuclear - transplantation; Lung - diagnostic imaging; Lung - pathology; Lung Diseases, Interstitial - diagnostic imaging; Lung Diseases, Interstitial - pathology; Lung Diseases, Interstitial - therapy; Lungs; Sampling; Sulfates; Swine; Therapy; Tomography, X-Ray Computed; Vessels
• ISSN: 1059-910X; 1097-0029; 1097-0029
• DOI: 10.1002/jemt.20981

Parte superior do formulário Digite um texto ou endereço de um site ou traduza um documento. The aim of this study is to evaluate the histological changes in lung parenchyma of pigs affected by interstitial lung disease induced after the infusion of bone marrow mononuclear cells (BMMCs). Ten female swines were submitted to pulmonary fibrosis induced by a single dose of intratracheal bleomicine sulfate. Animals were arranged into two groups: Group 1: induced‐disease control and Group 2: cell therapy using BMMCs. Both groups were clinically evaluated for 180 days. High‐resolution computed tomography (HRCT) was performed at 90 and 180 days. BMMC sampling was performed in cell therapy group at 90 days. Euthanasia was performed, and samples were collected for histology and immunohistochemistry. The 90‐days HRCT demonstrated typical interstitial lesions in pulmonary parenchyma similarly to human disease. The 180‐days HRCT in Group 1 demonstrated advanced stages of the disease when compared with Group 2. Immunohistochemistry analysis suggests the presence of pre‐existent vessels and neoformed vessels as well as predominant young cells in the injured parenchyma of Group 2. Immunohistochemistry analysis suggests that cell therapy would promote a reconstructive response. Histology and HRCT analysis suggest a positive application of swine as a model for a bleomicine inducing of fibrotic interstitial pulmonary disease. Microsc. Res. Tech., 2011. © 2011 Wiley‐Liss, Inc.