Intraspecific genetic variability of Edwardsiella tarda strains from cultured turbot

Edwardsiella tarda is an enterobacterial fish pathogen that causes mortality in various fish species worldwide. In this study, we analyzed the intraspecific variability in a collection of E. tarda strains isolated from turbot. To do this we employed 4 polymerase chain reaction (PCR)-based methods: (...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2011-07, Vol.95 (3), p.253-258
Main Authors: CASTRO, N, TORANZO, A. E, BASTARDO, A, BARJA, J. L, MAGARINOS, B
Format: Article
Language:English
Subjects:
Animal aquaculture
Animal productions
Animals
Biological and medical sciences
Edwardsiella tarda
Edwardsiella tarda - genetics
Enterobacteriaceae Infections - microbiology
Enterobacteriaceae Infections - veterinary
Fish Diseases - microbiology
Flatfishes
Fundamental and applied biological sciences. Psychology
Genetic Variation
Marine
Phylogeny
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Random Amplified Polymorphic DNA Technique - methods
Random Amplified Polymorphic DNA Technique - veterinary
Scophthalmus maximus
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Summary:Edwardsiella tarda is an enterobacterial fish pathogen that causes mortality in various fish species worldwide. In this study, we analyzed the intraspecific variability in a collection of E. tarda strains isolated from turbot. To do this we employed 4 polymerase chain reaction (PCR)-based methods: (1) random amplified polymorphic DNA (RAPD), (2) enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), (3) repetitive extragenic palindromic-PCR (REP-PCR) and (4) BOX-PCR. E. tarda isolates from different hosts were also included for comparison. E. tarda strains from turbot showed high molecular homogeneity when RAPD (primers P3 and P6), ERIC-PCR and BOX-PCR were employed. However, with regard to the REP-PCR and RAPD (primers P4 and P5) techniques, different genetic groups could be established within these isolates using either technique. The 2 RAPD types presented an 85% similarity, while those obtained with REP-PCR showed 74% similarity. Based on the results obtained, although a high genetic homogeneity was found in turbot isolates, the RAPD test (with primers P4 and P5) and REP-PCR were capable of discrimination within these strains, and they are therefore considered the most appropriate typing methods for studies of edwardsiellosis in turbot.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao02363