Diverse Metabolic Profiles of a Streptomyces Strain Isolated from a Hyper-arid Environment

• Published in: Journal of natural products (Washington, D.C.) D.C.), 2011-09, Vol.74 (9), p.1965-1971
• Main Authors: Rateb, Mostafa E, Houssen, Wael E, Harrison, William T. A, Deng, Hai, Okoro, Chinyere K, Asenjo, Juan A, Andrews, Barbara A, Bull, Alan T, Goodfellow, Michael, Ebel, Rainer, Jaspars, Marcel
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Bacillus subtilis - drug effects; Cinnamates - chemistry; Cinnamates - isolation & purification; Escherichia coli - drug effects; Hygromycin B - analogs & derivatives; Hygromycin B - chemistry; Hygromycin B - isolation & purification; Listeria monocytogenes - drug effects; Macrolides - chemistry; Macrolides - isolation & purification; Macrolides - pharmacology; Metabolome; Microbial Sensitivity Tests; Molecular Structure; Staphylococcus aureus - drug effects; Streptomyces; Streptomyces - chemistry; Streptomyces - genetics; Streptomyces - metabolism; Vibrio - drug effects
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np200470u

The metabolic profile of Streptomyces sp. strain C34, isolated from the Chilean hyper-arid Atacama Desert soil, is dependent on the culture media used for its growth. The application of an OSMAC approach on this strain using a range of cultivation media resulted in the isolation and identification of three new compounds from the rare class of 22-membered macrolactone polyketides, named chaxalactins A–C (1–3). In addition, the known compounds deferroxamine E (4), hygromycin A (5), and 5″-dihydrohygromycin A (6) were detected. The isolated compounds were characterized by NMR spectroscopy and accurate mass spectrometric analysis. Compounds 1–3 displayed strong activity against Gram-positive but weak activity Gram-negative strains tested.