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PET Imaging of Hypoxia-Inducible Factor-1-Active Tumor Cells with Pretargeted Oxygen-Dependent Degradable Streptavidin and a Novel 18F-Labeled Biotin Derivative
Purpose We aimed to evaluate the feasibility of using streptavidin–biotin-based pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor (HIF)-1-active tumors. Procedures We used POS, a genetically engineered form of streptavidin that selectively stabilizes in HIF-1-ac...
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| Published in: | Molecular imaging and biology 2011-10, Vol.13 (5), p.1003-1010 |
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| Main Authors: | , , , , , , , , , , |
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| container_title | Molecular imaging and biology |
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| creator | Kudo, Takashi Ueda, Masashi Konishi, Hiroaki Kawashima, Hidekazu Kuge, Yuji Mukai, Takahiro Miyano, Azusa Tanaka, Shotaro Kizaka-Kondoh, Shinae Hiraoka, Masahiro Saji, Hideo |
| description | Purpose
We aimed to evaluate the feasibility of using streptavidin–biotin-based pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor (HIF)-1-active tumors.
Procedures
We used POS, a genetically engineered form of streptavidin that selectively stabilizes in HIF-1-active cells, and (4-
18
F-fluorobenzoyl)norbiotinamide (
18
F-FBB), a radiolabeled biotin derivative, for performing a biodistribution study and for PET imaging. The tumoral
18
F-FBB accumulation was compared to the HIF-1-dependent luciferase bioluminescence and HIF-1α immunohistochemical signal.
Results
18
F-FBB accumulation was observed in POS-pretargeted tumors in mice (2.85 ± 0.55% injected dose per gram at 3 h), and clear PET images were obtained at the same time point. The tumoral
18
F-FBB accumulation positively correlated with luciferase bioluminescence (
R
= 0.72,
P
|
| doi_str_mv | 10.1007/s11307-010-0418-6 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_894814578</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>894814578</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2316-7bc6f5f8ed57c4d26631933740623be7495e40ed5a3a0dc3131c326bda8371d83</originalsourceid><addsrcrecordid>eNp9kc1u1DAURiMEoqXwAGyQd6wM_kkcz7LMdOhII1qJYW059k1wlTjBdqadt-FRcTSFJStf6fvusa5OUbyn5BMlpP4cKeWkxoQSTEoqsXhRXFIpCGaEsJd5rrjAVHB2UbyJ8YEQWlPGXxcXjEguV0ReFr_vbw5oN-jO-Q6NLbo9TeOT03jn7Wxc0wPaapPGgCm-NskdAR3mYQxoDX0f0aNLP9F9gKRDBwksuns6deDxBibwFnxCG-iCtnoBfU8BpqSPzjqPtLdIo2_jEXpE5RbvdQN9BnxxY8rxBoI76uW_t8WrVvcR3j2_V8WP7c1hfYv3d1936-s9NoxTgevGiLZqJdiqNqVlQnC64rwuiWC8gbpcVVCSnGquiTWccmo4E43VktfUSn5VfDxzpzD-miEmNbho8pXawzhHJVelpGVVL016bpowxhigVVNwgw4nRYlavKizF5W9qMWLEnnnwzN9bgaw_zb-isgFdi7EHPkOgnoY5-Dzxf-h_gH_YJif</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>894814578</pqid></control><display><type>article</type><title>PET Imaging of Hypoxia-Inducible Factor-1-Active Tumor Cells with Pretargeted Oxygen-Dependent Degradable Streptavidin and a Novel 18F-Labeled Biotin Derivative</title><source>Springer Link</source><creator>Kudo, Takashi ; Ueda, Masashi ; Konishi, Hiroaki ; Kawashima, Hidekazu ; Kuge, Yuji ; Mukai, Takahiro ; Miyano, Azusa ; Tanaka, Shotaro ; Kizaka-Kondoh, Shinae ; Hiraoka, Masahiro ; Saji, Hideo</creator><creatorcontrib>Kudo, Takashi ; Ueda, Masashi ; Konishi, Hiroaki ; Kawashima, Hidekazu ; Kuge, Yuji ; Mukai, Takahiro ; Miyano, Azusa ; Tanaka, Shotaro ; Kizaka-Kondoh, Shinae ; Hiraoka, Masahiro ; Saji, Hideo</creatorcontrib><description>Purpose
We aimed to evaluate the feasibility of using streptavidin–biotin-based pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor (HIF)-1-active tumors.
Procedures
We used POS, a genetically engineered form of streptavidin that selectively stabilizes in HIF-1-active cells, and (4-
18
F-fluorobenzoyl)norbiotinamide (
18
F-FBB), a radiolabeled biotin derivative, for performing a biodistribution study and for PET imaging. The tumoral
18
F-FBB accumulation was compared to the HIF-1-dependent luciferase bioluminescence and HIF-1α immunohistochemical signal.
Results
18
F-FBB accumulation was observed in POS-pretargeted tumors in mice (2.85 ± 0.55% injected dose per gram at 3 h), and clear PET images were obtained at the same time point. The tumoral
18
F-FBB accumulation positively correlated with luciferase bioluminescence (
R
= 0.72,
P
< 0.05), and most of the area showing
18
F-FBB accumulation corresponded to HIF-1α-positive areas.
Conclusion
Pretargeting with POS and
18
F-FBB is an effective approach for PET imaging of HIF-1-active areas in tumors.</description><identifier>ISSN: 1536-1632</identifier><identifier>EISSN: 1860-2002</identifier><identifier>DOI: 10.1007/s11307-010-0418-6</identifier><identifier>PMID: 20838908</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Animals ; Biotin - metabolism ; Cell Line, Tumor ; Fluorine Radioisotopes - metabolism ; Hypoxia-Inducible Factor 1 - metabolism ; Imaging ; Immunohistochemistry ; Medicine ; Medicine & Public Health ; Mice ; Oxygen - metabolism ; Positron-Emission Tomography ; Radiology ; Research Article ; Streptavidin</subject><ispartof>Molecular imaging and biology, 2011-10, Vol.13 (5), p.1003-1010</ispartof><rights>Academy of Molecular Imaging and Society for Molecular Imaging 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2316-7bc6f5f8ed57c4d26631933740623be7495e40ed5a3a0dc3131c326bda8371d83</citedby><cites>FETCH-LOGICAL-c2316-7bc6f5f8ed57c4d26631933740623be7495e40ed5a3a0dc3131c326bda8371d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20838908$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kudo, Takashi</creatorcontrib><creatorcontrib>Ueda, Masashi</creatorcontrib><creatorcontrib>Konishi, Hiroaki</creatorcontrib><creatorcontrib>Kawashima, Hidekazu</creatorcontrib><creatorcontrib>Kuge, Yuji</creatorcontrib><creatorcontrib>Mukai, Takahiro</creatorcontrib><creatorcontrib>Miyano, Azusa</creatorcontrib><creatorcontrib>Tanaka, Shotaro</creatorcontrib><creatorcontrib>Kizaka-Kondoh, Shinae</creatorcontrib><creatorcontrib>Hiraoka, Masahiro</creatorcontrib><creatorcontrib>Saji, Hideo</creatorcontrib><title>PET Imaging of Hypoxia-Inducible Factor-1-Active Tumor Cells with Pretargeted Oxygen-Dependent Degradable Streptavidin and a Novel 18F-Labeled Biotin Derivative</title><title>Molecular imaging and biology</title><addtitle>Mol Imaging Biol</addtitle><addtitle>Mol Imaging Biol</addtitle><description>Purpose
We aimed to evaluate the feasibility of using streptavidin–biotin-based pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor (HIF)-1-active tumors.
Procedures
We used POS, a genetically engineered form of streptavidin that selectively stabilizes in HIF-1-active cells, and (4-
18
F-fluorobenzoyl)norbiotinamide (
18
F-FBB), a radiolabeled biotin derivative, for performing a biodistribution study and for PET imaging. The tumoral
18
F-FBB accumulation was compared to the HIF-1-dependent luciferase bioluminescence and HIF-1α immunohistochemical signal.
Results
18
F-FBB accumulation was observed in POS-pretargeted tumors in mice (2.85 ± 0.55% injected dose per gram at 3 h), and clear PET images were obtained at the same time point. The tumoral
18
F-FBB accumulation positively correlated with luciferase bioluminescence (
R
= 0.72,
P
< 0.05), and most of the area showing
18
F-FBB accumulation corresponded to HIF-1α-positive areas.
Conclusion
Pretargeting with POS and
18
F-FBB is an effective approach for PET imaging of HIF-1-active areas in tumors.</description><subject>Animals</subject><subject>Biotin - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Fluorine Radioisotopes - metabolism</subject><subject>Hypoxia-Inducible Factor 1 - metabolism</subject><subject>Imaging</subject><subject>Immunohistochemistry</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Oxygen - metabolism</subject><subject>Positron-Emission Tomography</subject><subject>Radiology</subject><subject>Research Article</subject><subject>Streptavidin</subject><issn>1536-1632</issn><issn>1860-2002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u1DAURiMEoqXwAGyQd6wM_kkcz7LMdOhII1qJYW059k1wlTjBdqadt-FRcTSFJStf6fvusa5OUbyn5BMlpP4cKeWkxoQSTEoqsXhRXFIpCGaEsJd5rrjAVHB2UbyJ8YEQWlPGXxcXjEguV0ReFr_vbw5oN-jO-Q6NLbo9TeOT03jn7Wxc0wPaapPGgCm-NskdAR3mYQxoDX0f0aNLP9F9gKRDBwksuns6deDxBibwFnxCG-iCtnoBfU8BpqSPzjqPtLdIo2_jEXpE5RbvdQN9BnxxY8rxBoI76uW_t8WrVvcR3j2_V8WP7c1hfYv3d1936-s9NoxTgevGiLZqJdiqNqVlQnC64rwuiWC8gbpcVVCSnGquiTWccmo4E43VktfUSn5VfDxzpzD-miEmNbho8pXawzhHJVelpGVVL016bpowxhigVVNwgw4nRYlavKizF5W9qMWLEnnnwzN9bgaw_zb-isgFdi7EHPkOgnoY5-Dzxf-h_gH_YJif</recordid><startdate>201110</startdate><enddate>201110</enddate><creator>Kudo, Takashi</creator><creator>Ueda, Masashi</creator><creator>Konishi, Hiroaki</creator><creator>Kawashima, Hidekazu</creator><creator>Kuge, Yuji</creator><creator>Mukai, Takahiro</creator><creator>Miyano, Azusa</creator><creator>Tanaka, Shotaro</creator><creator>Kizaka-Kondoh, Shinae</creator><creator>Hiraoka, Masahiro</creator><creator>Saji, Hideo</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201110</creationdate><title>PET Imaging of Hypoxia-Inducible Factor-1-Active Tumor Cells with Pretargeted Oxygen-Dependent Degradable Streptavidin and a Novel 18F-Labeled Biotin Derivative</title><author>Kudo, Takashi ; Ueda, Masashi ; Konishi, Hiroaki ; Kawashima, Hidekazu ; Kuge, Yuji ; Mukai, Takahiro ; Miyano, Azusa ; Tanaka, Shotaro ; Kizaka-Kondoh, Shinae ; Hiraoka, Masahiro ; Saji, Hideo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2316-7bc6f5f8ed57c4d26631933740623be7495e40ed5a3a0dc3131c326bda8371d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biotin - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Fluorine Radioisotopes - metabolism</topic><topic>Hypoxia-Inducible Factor 1 - metabolism</topic><topic>Imaging</topic><topic>Immunohistochemistry</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Oxygen - metabolism</topic><topic>Positron-Emission Tomography</topic><topic>Radiology</topic><topic>Research Article</topic><topic>Streptavidin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kudo, Takashi</creatorcontrib><creatorcontrib>Ueda, Masashi</creatorcontrib><creatorcontrib>Konishi, Hiroaki</creatorcontrib><creatorcontrib>Kawashima, Hidekazu</creatorcontrib><creatorcontrib>Kuge, Yuji</creatorcontrib><creatorcontrib>Mukai, Takahiro</creatorcontrib><creatorcontrib>Miyano, Azusa</creatorcontrib><creatorcontrib>Tanaka, Shotaro</creatorcontrib><creatorcontrib>Kizaka-Kondoh, Shinae</creatorcontrib><creatorcontrib>Hiraoka, Masahiro</creatorcontrib><creatorcontrib>Saji, Hideo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular imaging and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kudo, Takashi</au><au>Ueda, Masashi</au><au>Konishi, Hiroaki</au><au>Kawashima, Hidekazu</au><au>Kuge, Yuji</au><au>Mukai, Takahiro</au><au>Miyano, Azusa</au><au>Tanaka, Shotaro</au><au>Kizaka-Kondoh, Shinae</au><au>Hiraoka, Masahiro</au><au>Saji, Hideo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PET Imaging of Hypoxia-Inducible Factor-1-Active Tumor Cells with Pretargeted Oxygen-Dependent Degradable Streptavidin and a Novel 18F-Labeled Biotin Derivative</atitle><jtitle>Molecular imaging and biology</jtitle><stitle>Mol Imaging Biol</stitle><addtitle>Mol Imaging Biol</addtitle><date>2011-10</date><risdate>2011</risdate><volume>13</volume><issue>5</issue><spage>1003</spage><epage>1010</epage><pages>1003-1010</pages><issn>1536-1632</issn><eissn>1860-2002</eissn><abstract>Purpose
We aimed to evaluate the feasibility of using streptavidin–biotin-based pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor (HIF)-1-active tumors.
Procedures
We used POS, a genetically engineered form of streptavidin that selectively stabilizes in HIF-1-active cells, and (4-
18
F-fluorobenzoyl)norbiotinamide (
18
F-FBB), a radiolabeled biotin derivative, for performing a biodistribution study and for PET imaging. The tumoral
18
F-FBB accumulation was compared to the HIF-1-dependent luciferase bioluminescence and HIF-1α immunohistochemical signal.
Results
18
F-FBB accumulation was observed in POS-pretargeted tumors in mice (2.85 ± 0.55% injected dose per gram at 3 h), and clear PET images were obtained at the same time point. The tumoral
18
F-FBB accumulation positively correlated with luciferase bioluminescence (
R
= 0.72,
P
< 0.05), and most of the area showing
18
F-FBB accumulation corresponded to HIF-1α-positive areas.
Conclusion
Pretargeting with POS and
18
F-FBB is an effective approach for PET imaging of HIF-1-active areas in tumors.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>20838908</pmid><doi>10.1007/s11307-010-0418-6</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biotin - metabolism Cell Line, Tumor Fluorine Radioisotopes - metabolism Hypoxia-Inducible Factor 1 - metabolism Imaging Immunohistochemistry Medicine Medicine & Public Health Mice Oxygen - metabolism Positron-Emission Tomography Radiology Research Article Streptavidin |
| title | PET Imaging of Hypoxia-Inducible Factor-1-Active Tumor Cells with Pretargeted Oxygen-Dependent Degradable Streptavidin and a Novel 18F-Labeled Biotin Derivative |
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