Analysis of the expression of platelet antigens CD9 and CD41/61 in transgenic rabbits with the integrated human blood clotting factor VIII gene construct

The objective of this research was to study the expression of cell membrane molecules CD9 and CD41/61 of transgenic rabbit with integrated human factor VIII (rhFVIII) gene construct. The expressions of these molecules have been monitored during two lactations of transgenic rabbits and simultaneously...

Full description

Saved in:
Bibliographic Details
Published in:General physiology and biophysics 2011, Vol.30 Spec No, p.S83-S87
Main Authors: Simon, Michal, Antalikova, Jana, Chrenek, Peter, Horovska, Lubica, Hluchy, Svatoslav, Michalkova, Katarina, Jankovicova, Jana, Tancin, Vladimir
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Antigens, CD - metabolism
Blood Coagulation - genetics
Blood Platelets - metabolism
Cell Line
Factor VIII - genetics
Female
Gene Expression Regulation - genetics
Humans
Integrin beta3 - metabolism
Membrane Glycoproteins - metabolism
Platelet Membrane Glycoprotein IIb - metabolism
Rabbits
Tetraspanin-29
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of this research was to study the expression of cell membrane molecules CD9 and CD41/61 of transgenic rabbit with integrated human factor VIII (rhFVIII) gene construct. The expressions of these molecules have been monitored during two lactations of transgenic rabbits and simultaneously compared with the expression of the same molecules of non-transgenic rabbits. The immunochemical analysis by indirect immunofluorescence, ELISA and indirect immunoperoxidase staining of blood cells and udder tissues show that the insertion of the WAP-hFVIII gene construct into the rabbit genome, do not influence the expression of cell membrane antigens CD9 and CD41/61 on the blood platelets, polymorphonuclear blood cells, milk somatic cells and mammary gland tissues.
ISSN:0231-5882
DOI:10.4149/gpb_2011_SI1_83