Capillary electrophoresis immunoassays with conjugated quantum dots

Water‐soluble CdTe quantum dots (QDs) and their conjugates with antibodies and antigenes were prepared by optimized procedures for applications in CE immunoassays. The QD size of 3.5 nm, excitation spectrum in the range of 300–500 nm, the maximum wavelength of the emission spectrum at 610 nm, quantu...

Full description

Saved in:
Bibliographic Details
Published in:Electrophoresis 2011-05, Vol.32 (10), p.1217-1223
Main Authors: Klepárník, Karel, Voráčová, Ivona, Lišková, Marcela, Přikryl, Jan, Hezinová, Věra, Foret, František
Format: Article
Language:English
Subjects:
Cadmium Compounds - chemistry
Cadmium tellurides
Conjugates
Electrophoresis
Electrophoresis, Capillary - methods
Immunoassay
Immunoassay - methods
LIF
Luminescence
Luminescent Measurements
Optimization
Ovalbumin - chemistry
Particle Size
Quantum Dots
Tellurium - chemistry
Wavelengths
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Water‐soluble CdTe quantum dots (QDs) and their conjugates with antibodies and antigenes were prepared by optimized procedures for applications in CE immunoassays. The QD size of 3.5 nm, excitation spectrum in the range of 300–500 nm, the maximum wavelength of the emission spectrum at 610 nm, quantum yield of 0.25 and luminescence lifetimes in the range of 3.6–43 ns were determined. The 0.1 M solution of TRIS/TAPS (pH 8.3) was found to be the optimum buffer for the separation of the antiovalbumin–ovalbumin immunocomplex from the free conjugates of QDs.
ISSN:0173-0835
1522-2683
1522-2683
DOI:10.1002/elps.201000652