T3 acutely increases GH mRNA translation rate and GH secretion in hypothyroid rats

Cytoskeleton controls the stability of transcripts, by mechanisms that involve mRNAs and eEF1A attachment to it. Besides, it plays a key role in protein synthesis and secretion, which seems to be impaired in somatotrophs of hypothyroid rats, whose cytoskeleton is disarranged. This study investigated...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2010-04, Vol.317 (1), p.1-7
Main Authors: da Silva, F. Goulart, Giannocco, G., Luchessi, A.D., Curi, R., Nunes, M.T.
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Body Weight - drug effects
Cytoskeleton
Cytoskeleton - drug effects
Cytoskeleton - metabolism
eEF1A
Gene Expression Regulation - drug effects
Growth Hormone - genetics
Growth Hormone - secretion
Hypothyroidism - genetics
Hypothyroidism - pathology
Insulin-Like Growth Factor I - genetics
Insulin-Like Growth Factor I - metabolism
Liver - drug effects
Liver - metabolism
Liver - pathology
Male
Nongenomic action
Organ Size - drug effects
Peptide Elongation Factor 1 - metabolism
Pituitary Gland - drug effects
Pituitary Gland - metabolism
Pituitary Gland - pathology
Polyribosomes - drug effects
Polyribosomes - metabolism
Polysomes
Protein Binding - drug effects
Protein Biosynthesis - drug effects
Rats
Rats, Wistar
RNA, Messenger - genetics
RNA, Messenger - metabolism
Triiodothyronine - pharmacology
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Summary:Cytoskeleton controls the stability of transcripts, by mechanisms that involve mRNAs and eEF1A attachment to it. Besides, it plays a key role in protein synthesis and secretion, which seems to be impaired in somatotrophs of hypothyroid rats, whose cytoskeleton is disarranged. This study investigated the: eEF1A and GH mRNA binding to cytoskeleton plus GH mRNA translation rate and GH secretion, in sham-operated and thyroidectomized rats treated with T3 or saline, and killed 30 min thereafter. Thyroidectomy reduced: (a) pituitary F-actin content, and eEF1A plus GH mRNA binding to it; (b) GH mRNA recruitment to polysome; and (c) liver IGF-I mRNA expression, indicating that GH mRNA stability and translation rate, as well as GH secretion were impaired. T3 acutely reversed all these changes, which points toward a nongenomic action of T3 on cytoskeleton rearrangement, which might contribute to the increase on GH mRNA translation rate and GH secretion.
ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2009.12.005