Morphological characterization of the growing front of Rhizopus oligosporus in solid media

Rhizopus oligosporus is used to prepare tempeh and is formed by hyphae which grow as a complex branched structure with irregular borders. The objective of this research was to characterize the macro and micro structural development of R. oligosporus colonies growing on solid media in Petri dishes th...

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Bibliographic Details
Published in:Journal of food engineering 2010-12, Vol.101 (3), p.309-317
Main Authors: Díaz, Brenda Hildeliza Camacho, Aparicio, Antonio Jiménez, Chanona-Pérez, José Jorge, Calderón-Domínguez, Georgina, Alamilla-Beltrán, Liliana, Hernández-Sánchez, Humberto, Gutiérrez-López, Gustavo F.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Borders
Colonies
dry matter accumulation
Food engineering
Food industries
Fractal analysis
Fractal dimension
fractal dimensions
Fractals
Fundamental and applied biological sciences. Psychology
Fungus skeletonized images
General aspects
growing fronts
hyphae
image analysis
Image processing
microbial growth
microstructure
Oscillating growing of fungus
R. oligosporus growing front
Rhizopus microsporus var. oligosporus
Rhizopus oligosporus
starter cultures
Surface layer
tempeh
Texture
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Summary:Rhizopus oligosporus is used to prepare tempeh and is formed by hyphae which grow as a complex branched structure with irregular borders. The objective of this research was to characterize the macro and micro structural development of R. oligosporus colonies growing on solid media in Petri dishes through image processing and fractal dimension. Stereomicroscope images of the colony were used to detecting the zone in which the fractal dimension (FDE) of the edge (growing front) of the colony represented 97% of the maximum FDE for the 80% of the image of the border. Evaluated characteristics were: radial growth, number of tips in the growing front and the average length of hypha. Two simultaneous events of different and oscillating magnitude were detected in the growing front: branching and increase of the average hypha length. Biomass had a good correlation with radius of the colony, fractal dimension of texture (FDT) and number of tips in the border.
ISSN:0260-8774
1873-5770
DOI:10.1016/j.jfoodeng.2010.06.028