Quantification of plasma lactate concentrations using capillary electrophoresis with contactless conductivity detection

• Published in: Electrophoresis 2011-04, Vol.32 (8), p.884-889
• Main Authors: Pormsila, Worapan, Morand, Réjane, Krähenbühl, Stephan, Hauser, Peter C.
• Format: Article
• Language: English
• Subjects: Acetonitriles - chemistry; Buffers; Capillarity; Contactless conductivity detection; Correlation analysis; Electric Conductivity; Electrophoresis; Electrophoresis, Capillary - methods; Exercise - physiology; Humans; Lactate; Lactate threshold; Lactates; Lactic Acid - blood; Linear Models; Methanol - chemistry; Methyl alcohol; Plasma lactate; Reproducibility of Results; Running; Sensitivity and Specificity; Thresholds
• ISSN: 0173-0835; 1522-2683; 1522-2683
• DOI: 10.1002/elps.201000420

The quantification of plasma lactate and evaluation of the lactate threshold by CE with capacitively coupled contactless conductivity is demonstrated. The only sample preparation needed was deproteinization with a ACN/methanol mixture. A solution of 10 mmol/L 2‐morpholinoethanesulfonic acid monohydrate, 10 mmol/L DL‐histidine, 70 μmol/L hexadecyltrimethylammonium bromide, pH 6.0 was found suitable as running buffer. Linearity was achieved for the concentration range of 10–1000 μmol/L with a correlation coefficient of 0.9994. The limit of detection (3 S/N) was determined as 3.2 μmol/L. Intra‐ and inter‐day variabilities were less than 7% RSD. The suitability of the method could be demonstrated by analyzing various clinical samples, where the results correlated satisfactorily with those of an established enzymatic method.