Enantioseparation of α-amino acids on an 18-crown-6-tetracarboxylic acid-bonded silica by capillary electrochromatography

(−)-(18-Crown-6)-2,3,11,12-tetracarboxylic acid-bonded silica was used as the chiral stationary phase in capillary electrochromatography (CEC) for enantioseparation of some α-amino acids. Separation data in CEC were measured in mobile phases of varying pH, and composition of methanol and buffer, and...

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Bibliographic Details
Published in:Journal of Chromatography A 2010-02, Vol.1217 (8), p.1425-1428
Main Authors: Lee, Taeho, Lee, Wonjae, Hyun, Myung Ho, Park, Jung Hag
Format: Article
Language:English
Subjects:
18-Crown-6-tetracarboxylic acid-bonded silica
Amino acids
Analytical chemistry
Buffers
Capillarity
CEC
Chemistry
Chromatographic methods and physical methods associated with chromatography
Concentration (composition)
Electrochromatography
Enantioseparation
Exact sciences and technology
Methyl alcohol
Other chromatographic methods
Silicon dioxide
α-Amino acids
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Description
Summary:(−)-(18-Crown-6)-2,3,11,12-tetracarboxylic acid-bonded silica was used as the chiral stationary phase in capillary electrochromatography (CEC) for enantioseparation of some α-amino acids. Separation data in CEC were measured in mobile phases of varying pH, and composition of methanol and buffer, and compared with those in capillary liquid chromatography (CLC). In CEC better enantioseparation was generally obtained in the eluent of lower pH, higher buffer concentration and intermediate MeOH content, usually at the expense of analysis time. CEC showed generally better enantioselectivity and resolutions than CLC for the amino acids investigated.
ISSN:0021-9673
DOI:10.1016/j.chroma.2009.12.064