Quantification of drugs of abuse and some stimulants in hair samples by liquid chromatography–electrospray ionization ion trap mass spectrometry

A qualitative and quantitative method for the analysis of drugs of abuse (cocaine and benzoylecgonine, opiates) and some stimulants in human hair was developed and validated. Hair samples were incubated with phosphate buffer (pH 5.0), chosen as the extraction medium, extracted with Bond Elut Certify...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-12, Vol.877 (31), p.3931-3936
Main Authors: Bucelli, Francesca, Fratini, Angela, Bavazzano, Paolo, Comodo, Nicola
Format: Article
Language:English
Subjects:
Analgesics, Opioid - analysis
Analysis
Analytical, structural and metabolic biochemistry
Antidoping
Biological and medical sciences
Cartridges
Chromatography
Chromatography, Liquid - methods
Cocaine - analogs & derivatives
Cocaine - analysis
Drugs
Drugs of abuse
Extraction
Fundamental and applied biological sciences. Psychology
General pharmacology
Hair
Hair - chemistry
Humans
Ion trap mass spectrometer
Ionization
Limit of Detection
Linearity
Liquid chromatography–mass spectrometry
Medical sciences
Pharmacology. Drug treatments
Spectrometry, Mass, Electrospray Ionization - methods
Stimulants
Street Drugs - analysis
Substance Abuse Detection - methods
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Summary:A qualitative and quantitative method for the analysis of drugs of abuse (cocaine and benzoylecgonine, opiates) and some stimulants in human hair was developed and validated. Hair samples were incubated with phosphate buffer (pH 5.0), chosen as the extraction medium, extracted with Bond Elut Certify cartridges and analyzed by LC–MS–MS and LC–MS 3 as confirmation for positive results. The method proved to be specific, accurate and precise across the calibration range (0.1–30 ng/mg) where good linearity was observed. Total extraction recovery, intra-assay accuracy and precision, limits of detection and limits of quantitation were estimated. The method was successfully applied to the analysis of hair samples collected from drug abusers and it was suitable for routine analytical applications in the Antidoping Laboratory of Public Health Laboratory.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2009.09.026