Recognition of immunogold markers in electron micrographs

Immunoelectron microscopy is used in cell biological research to study the spatial distribution of intracellular macromolecules at the ultrastructural level. Colloidal gold particles (immunogold markers) are commonly used to localise molecules of interest on ultrathin sections and can be visualised...

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Bibliographic Details
Published in:Journal of structural biology 2011-11, Vol.176 (2), p.151-158
Main Authors: Wang, Ruixuan, Pokhariya, Himanshu, McKenna, Stephen J., Lucocq, John
Format: Article
Language:English
Subjects:
Algorithms
Biomarkers - metabolism
Cryoultramicrotomy
Golgi Apparatus - metabolism
Golgi Apparatus - ultrastructure
Image Processing, Computer-Assisted - methods
Immunoelectron microscopy
Immunogold markers
Immunohistochemistry - methods
Lysosomes - metabolism
Lysosomes - ultrastructure
Microscopy, Immunoelectron - methods
Reproducibility of Results
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Summary:Immunoelectron microscopy is used in cell biological research to study the spatial distribution of intracellular macromolecules at the ultrastructural level. Colloidal gold particles (immunogold markers) are commonly used to localise molecules of interest on ultrathin sections and can be visualised in transmission electron micrographs as dark spots. Quantitative analysis involves detection of the immunogold markers, and is often performed manually or interactively as part of a stereological estimation technique. The method presented in this paper automatically detects and counts immunogold markers, estimating the location, size and type of each marker. It was evaluated on single-labelled as well as double-labelled images showing markers of two different sizes. This is a first step towards automatic analysis of immunoelectron micrographs, enabling a rapid and more complete quantitative analysis than is currently practicable.
ISSN:1047-8477
1095-8657
DOI:10.1016/j.jsb.2011.07.005