Identification of Anaplasma spp. Rickettsia Isolated from Horses from Clinical Disease Cases in Poland

Summary This study was aimed at determining the cause of the diseases in five horses exhibiting symptoms of fever, joint oedema and ataxia and thrombocytopenia. The PCR technique revealed the presence in the blood of 16S RNA Anaplasma/Ehrlichia spp. genetic material. DNA amplification with primers E...

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Bibliographic Details
Published in:Zoonoses and public health 2011-11, Vol.58 (7), p.514-518
Main Authors: Adaszek, Ł., Winiarczyk, S.
Format: Article
Language:English
Subjects:
Anaplasma
Anaplasma - classification
Anaplasma - isolation & purification
Anaplasma phagocytophilum
Anaplasmosis - drug therapy
Anaplasmosis - epidemiology
Anaplasmosis - microbiology
Animals
Anti-Bacterial Agents - therapeutic use
Antibiotics
Ataxia
Bacterial infections
horse
Horse Diseases - drug therapy
Horse Diseases - epidemiology
Horse Diseases - microbiology
Horses
Oxytetracycline - therapeutic use
PCR
Poland - epidemiology
Polymerase chain reaction
Polymerase Chain Reaction - veterinary
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
thrombocytopenia
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Summary:Summary This study was aimed at determining the cause of the diseases in five horses exhibiting symptoms of fever, joint oedema and ataxia and thrombocytopenia. The PCR technique revealed the presence in the blood of 16S RNA Anaplasma/Ehrlichia spp. genetic material. DNA amplification with primers EHR 521 and EHR 747 gave a product with a size of 247 bp. The sequence of the PCR product obtained showed a 97.6–99.6% similarity with a sequence of a fragment of 16S RNA Anaplasma phagocytophilum, gene number EU 090186 from GenBank. Intravenous administration of oxytetracycline at a dose of 8 mg/kg of body mass for 7 days resulted in a gradual recovery.
ISSN:1863-1959
1863-2378
DOI:10.1111/j.1863-2378.2011.01394.x