Characterization of Beauveria bassiana MsW1 isolated from pine sawyers, Monochamus saltuarius

Monochamus saltuarius is a vector for pine wilt disease that causes enormous damage to native pine trees in Korea. To develop a biological control method for this pine wilt disease vector, an entomopathogenic fungus was isolated from the cadaver of an adult M. saltuarius supporting fungal conidiatio...

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Bibliographic Details
Published in:Journal of basic microbiology 2011-10, Vol.51 (5), p.531-539
Main Authors: Shin, Tae Young, Choi, Jae Bang, Bae, Sung Min, Koo, Hyun Na, Roh, Jong Yul, Je, Yeon Ho, Jin, Byung Rae, Woo, Soo Dong
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Beauveria - classification
Beauveria - isolation & purification
Beauveria - pathogenicity
Beauveria bassiana
Coleoptera - microbiology
Entomopathogenic fungi
Genes, Fungal
Molecular Sequence Data
Monochamus saltuarius
Phylogeny
Sequence Alignment
Sequence Homology, Nucleic Acid
Virulence
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Summary:Monochamus saltuarius is a vector for pine wilt disease that causes enormous damage to native pine trees in Korea. To develop a biological control method for this pine wilt disease vector, an entomopathogenic fungus was isolated from the cadaver of an adult M. saltuarius supporting fungal conidiation. This fungus was named MsW1 and identified as Beauveria bassiana by microscopic examination, PCR amplification using B. bassiana ‐specific primers and genetic sequencing of the ITS and EF1‐α regions. Virulence tests against M. saltuarius were conducted with conidial suspensions (1 × 108 conidia/ml) of B. bassiana MsW1 in laboratory conditions. The median lethal times (LT50) of adults and larvae were 7.2 and 7 days, and 100% mortality was observed at 11 and 13 days after inoculation, respectively. This is the first characterization of B. bassiana from M. saltuarius. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201000348