Strains of Klebsiella pneumoniae Producing Extended Spectrum Beta-Lactamases, Isolated from Organ Recipients

• Published in: Transplantation proceedings 2011-10, Vol.43 (8), p.3128-3129
• Main Authors: Walter de Walthoffen, S, Mlynarczyk, A, Sawicka-Grzelak, A, Durlik, M, Paczek, L, Chmura, A, Ciszek, M, Chabros, L, Baczkowska, T, Mlynarczyk, G
• Format: Article
• Language: English
• Subjects: beta-Lactamases - biosynthesis; beta-Lactamases - genetics; Biological and medical sciences; Cross Infection - drug therapy; Cross Infection - etiology; Cross Infection - microbiology; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Klebsiella Infections - drug therapy; Klebsiella Infections - etiology; Klebsiella Infections - microbiology; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - enzymology; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - isolation & purification; Medical sciences; Microbial Sensitivity Tests; Polymerase Chain Reaction; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Tissue, organ and graft immunology; Transplants - adverse effects; Transplants - microbiology
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2011.08.003

Abstract Objective The aims of this work were to define the effectiveness of identification of the extended-spectrum beta-lactamases (ESBL) phenotype, and to define the genotype of Klebsiella pneumoniae β-lactamase. Materials and Methods We identified ESBL phenotypes in 110 strains of K pneumoniae isolated from samples from patients of transplantation wards, using the double-disk synergy test (DDST). For the chosen strains, polymerase chain reaction (PCR) was applied to detect genes determining SHV, CTX-M, and TEM. Results We showed synergism of clavulanic acid and investigated antibiotics including ceftazidime (89.1%), cefotaxime (80%), and aztreonam (82.7%) against ESBL-positive strains PCR revealed that TEM and CTX-M were present in 88.89% of strains. Conclusions The ESBL mechanism of resistance is frequent among K pneumoniae strains isolated from transplant recipients. Strains with simultaneous synthesis of more than one beta-lactamase predominated.