Hepatoprotective Effect of Prolactin and Cysteine Contained in Perfusion and Preservation Solutions on Porcine Liver Stored in Simple Hypothermia

Abstract Introduction The aim of our study was to determine the results of histidine-tryptophan-ketoglutarate (HTK) solutions modified by the addition of the antioxidant cysteine (Cys), and of prolactin (PRL) on storage of isolated porcine livers. Methods We measured in the media of isolated livers...

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Bibliographic Details
Published in:Transplantation proceedings 2011-10, Vol.43 (8), p.2882-2886
Main Authors: Ryszka, F, Dolińska, B, Caban, A, Ostróżka-Cieślik, A, Budziński, G, Krzysztofik, M, Oczkowicz, G, Cierpka, L
Format: Article
Language:English
Subjects:
Alanine Transaminase - metabolism
Animals
Aspartate Aminotransferases - metabolism
Biological and medical sciences
Cations - metabolism
Cold Ischemia
Cysteine - administration & dosage
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Glucose - chemistry
In Vitro Techniques
L-Lactate Dehydrogenase - metabolism
Lactic Acid - metabolism
Liver - drug effects
Liver - metabolism
Liver Transplantation
Mannitol - chemistry
Medical sciences
Organ Preservation - methods
Organ Preservation Solutions - chemistry
Perfusion
Potassium Chloride - chemistry
Procaine - chemistry
Prolactin - administration & dosage
Surgery
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Sus scrofa
Time Factors
Tissue, organ and graft immunology
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Summary:Abstract Introduction The aim of our study was to determine the results of histidine-tryptophan-ketoglutarate (HTK) solutions modified by the addition of the antioxidant cysteine (Cys), and of prolactin (PRL) on storage of isolated porcine livers. Methods We measured in the media of isolated livers stored for 24 hours in HTK (control group) or modified HTK + Cys (0.3 mmol/L) + PRL (3 IU/L study group) the amounts of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), lactic acid as well as Ca (II), Mg (II), Na (I) and K (I) ions during a 30-minute perfusion after 24 hours of storage. Results All tested markers were released more slowly into HTK + Cys + PRL with less release of K(I) and Mg(II) and greater of Na(I) and Ca(II) ions. Conclusions Addition of the Cys and PRL to HTK positively affected 24-hour storage of isolated livers.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2011.08.064