Hepatoprotective Effect of Prolactin and Cysteine Contained in Perfusion and Preservation Solutions on Porcine Liver Stored in Simple Hypothermia

• Published in: Transplantation proceedings 2011-10, Vol.43 (8), p.2882-2886
• Main Authors: Ryszka, F, Dolińska, B, Caban, A, Ostróżka-Cieślik, A, Budziński, G, Krzysztofik, M, Oczkowicz, G, Cierpka, L
• Format: Article
• Language: English
• Subjects: Alanine Transaminase - metabolism; Animals; Aspartate Aminotransferases - metabolism; Biological and medical sciences; Cations - metabolism; Cold Ischemia; Cysteine - administration & dosage; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glucose - chemistry; In Vitro Techniques; L-Lactate Dehydrogenase - metabolism; Lactic Acid - metabolism; Liver - drug effects; Liver - metabolism; Liver Transplantation; Mannitol - chemistry; Medical sciences; Organ Preservation - methods; Organ Preservation Solutions - chemistry; Perfusion; Potassium Chloride - chemistry; Procaine - chemistry; Prolactin - administration & dosage; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Sus scrofa; Time Factors; Tissue, organ and graft immunology
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2011.08.064

Abstract Introduction The aim of our study was to determine the results of histidine-tryptophan-ketoglutarate (HTK) solutions modified by the addition of the antioxidant cysteine (Cys), and of prolactin (PRL) on storage of isolated porcine livers. Methods We measured in the media of isolated livers stored for 24 hours in HTK (control group) or modified HTK + Cys (0.3 mmol/L) + PRL (3 IU/L study group) the amounts of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), lactic acid as well as Ca (II), Mg (II), Na (I) and K (I) ions during a 30-minute perfusion after 24 hours of storage. Results All tested markers were released more slowly into HTK + Cys + PRL with less release of K(I) and Mg(II) and greater of Na(I) and Ca(II) ions. Conclusions Addition of the Cys and PRL to HTK positively affected 24-hour storage of isolated livers.