Relationship between rumen methanogens and methane production in dairy cows fed diets supplemented with a feed enzyme additive

• Published in: Journal of applied microbiology 2011-11, Vol.111 (5), p.1148-1158
• Main Authors: Zhou, M, Chung, Y.‐H, Beauchemin, K.A, Holtshausen, L, Oba, M, McAllister, T.A, Guan, L.L
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cattle - microbiology; correlation; cow feeding; dairy; dairy cows; Denaturing Gradient Gel Electrophoresis; Diet - veterinary; Dietary Supplements; DNA, Bacterial - genetics; enzymatic hydrolysis; enzymes; Enzymes - administration & dosage; feed additives; feed supplements; Female; Fundamental and applied biological sciences. Psychology; Gene Dosage; hosts; Lactation; methane; Methane - biosynthesis; methane production; Methanobrevibacter - genetics; Methanobrevibacter - growth & development; Methanobrevibacter - isolation & purification; Methanobrevibacter smithii; methanogens; microbial communities; Microbiology; population; quantitative polymerase chain reaction; RNA, Ribosomal, 16S - genetics; rumen; Rumen - microbiology; Sequence Analysis, DNA
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2011.05126.x

Aims: To investigate the relationship between ruminal methanogen community and host enteric methane (CH4) production in lactating dairy cows fed diets supplemented with an exogenous fibrolytic enzyme additive. Methods and Results: Ecology of ruminal methanogens from dairy cows fed with or without exogenous fibrolytic enzymes was examined using PCR–denaturing gradient gel electrophoresis (PCR–DGGE) analyses and quantitative real‐time PCR (qRT‐PCR). The density of methanogens was not affected by the enzyme additive or sampling times, and no relationship was observed between the total methanogen population and CH4 yield (as g per head per day or g kg−1 DMI). The PCR–DGGE profiles consisted of 26 distinctive bands, with two bands similar to Methanogenic archaeon CH1270 negatively correlated, and one band similar to Methanobrevibacter gottschalkii strain HO positively correlated, with CH4 yield. Three bands similar to Methanogenic archaeon CH1270 or Methanobrevibacter smithii ATCC 35061 appeared after enzyme was added. Conclusions: Supplementing a dairy cow diet with an exogenous fibrolytic enzyme additive increased CH4 yield and altered the composition of the rumen methanogen community, but not the overall density of methanogens. Significance and Impact of the Study: This is the first study to identify the correlation between methanogen ecology and host CH4 yield from lactating dairy cows.