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Rhesus Monkey TRIM5α Has Distinct HIV-1 Restriction Activity Among Different Mammalian Cell Lines
Rhesus monkey TRIM5α (TRIM5α rh ), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears...
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| Published in: | Current microbiology 2011-12, Vol.63 (6), p.531-537 |
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| Main Authors: | , , , , |
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| Language: | English |
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| container_end_page | 537 |
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| container_title | Current microbiology |
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| creator | Gong, Jian Shen, Xi-Hui Qiu, Hui Chen, Chao Yang, Rong-Ge |
| description | Rhesus monkey TRIM5α (TRIM5α
rh
), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to some cellular cofactors. Here we reported that TRIM5α
rh
can significantly reduce the infection efficiency of VSV-G pseudotyped HIV-1/MA-YFP virus in human epithelial carcinoma (HeLa) cells, moderately reduce in porcine kidney (PK-15) cells and have no effect on the pseudotyped virus infection in Madin-Darby canine kidney (MDCK) cells. Furthermore, we found that the different HIV-1 restriction activities have no relation with the intracellular localization of TRIM5α
rh
. These results indicate that the cellular environment is very important for the efficient anti-HIV-1 activity of TRIM5α
rh
. We speculate that some unknown factors required for HIV-1 infection inhibition activity are adequately expressed in HeLa cells, inadequately expressed in PK-15 cells and absent in MDCK cells. |
| doi_str_mv | 10.1007/s00284-011-0009-z |
| format | article |
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rh
), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to some cellular cofactors. Here we reported that TRIM5α
rh
can significantly reduce the infection efficiency of VSV-G pseudotyped HIV-1/MA-YFP virus in human epithelial carcinoma (HeLa) cells, moderately reduce in porcine kidney (PK-15) cells and have no effect on the pseudotyped virus infection in Madin-Darby canine kidney (MDCK) cells. Furthermore, we found that the different HIV-1 restriction activities have no relation with the intracellular localization of TRIM5α
rh
. These results indicate that the cellular environment is very important for the efficient anti-HIV-1 activity of TRIM5α
rh
. We speculate that some unknown factors required for HIV-1 infection inhibition activity are adequately expressed in HeLa cells, inadequately expressed in PK-15 cells and absent in MDCK cells.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-011-0009-z</identifier><identifier>PMID: 21947260</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Amino Acid Sequence ; Animals ; Biomedical and Life Sciences ; Biotechnology ; Callithrix ; Cloning, Molecular ; Dogs ; Genetic Variation ; HeLa Cells ; HIV Infections - immunology ; HIV Infections - virology ; HIV-1 - genetics ; HIV-1 - immunology ; Humans ; Life Sciences ; Macaca mulatta ; Microbiology ; Microscopy, Confocal ; Microscopy, Fluorescence ; Molecular Sequence Data ; Proteins - genetics ; Proteins - immunology ; Species Specificity ; Transduction, Genetic</subject><ispartof>Current microbiology, 2011-12, Vol.63 (6), p.531-537</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-1ea3fd09b41cbd55bd93f653d02a91ab1706078f4d1e18d8a4e75d867de2f1623</citedby><cites>FETCH-LOGICAL-c343t-1ea3fd09b41cbd55bd93f653d02a91ab1706078f4d1e18d8a4e75d867de2f1623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21947260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gong, Jian</creatorcontrib><creatorcontrib>Shen, Xi-Hui</creatorcontrib><creatorcontrib>Qiu, Hui</creatorcontrib><creatorcontrib>Chen, Chao</creatorcontrib><creatorcontrib>Yang, Rong-Ge</creatorcontrib><title>Rhesus Monkey TRIM5α Has Distinct HIV-1 Restriction Activity Among Different Mammalian Cell Lines</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>Rhesus monkey TRIM5α (TRIM5α
rh
), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to some cellular cofactors. Here we reported that TRIM5α
rh
can significantly reduce the infection efficiency of VSV-G pseudotyped HIV-1/MA-YFP virus in human epithelial carcinoma (HeLa) cells, moderately reduce in porcine kidney (PK-15) cells and have no effect on the pseudotyped virus infection in Madin-Darby canine kidney (MDCK) cells. Furthermore, we found that the different HIV-1 restriction activities have no relation with the intracellular localization of TRIM5α
rh
. These results indicate that the cellular environment is very important for the efficient anti-HIV-1 activity of TRIM5α
rh
. We speculate that some unknown factors required for HIV-1 infection inhibition activity are adequately expressed in HeLa cells, inadequately expressed in PK-15 cells and absent in MDCK cells.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Callithrix</subject><subject>Cloning, Molecular</subject><subject>Dogs</subject><subject>Genetic Variation</subject><subject>HeLa Cells</subject><subject>HIV Infections - immunology</subject><subject>HIV Infections - virology</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - immunology</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Macaca mulatta</subject><subject>Microbiology</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Fluorescence</subject><subject>Molecular Sequence Data</subject><subject>Proteins - genetics</subject><subject>Proteins - immunology</subject><subject>Species Specificity</subject><subject>Transduction, Genetic</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kM9OGzEQh60K1AToA_RS-cbJMOP9Zx-jlDaRElWKgKvlXXvBIeul9m6l5K36In2mOgrl2NMc5puffvMR8hnhBgGq2wjARc4AkQGAZIcPZIp5xhlIiWdkClmeMVEWOCEXMW4BkEvAj2TCUeYVL2FK6s2zjWOk696_2D293yzXxZ_fdKEj_eri4Hwz0MXykSHd2DgE1wyu93SWxi837Oms6_1TItvWBusHutZdp3dOezq3ux1dOW_jFTlv9S7aT2_zkjx8u7ufL9jqx_flfLZiTao5MLQ6aw3IOsemNkVRG5m1ZZEZ4FqirrGCEirR5gYtCiN0bqvCiLIylrdY8uySXJ9yX0P_c0xtVedik2pob_sxKiGFyEFkZSLxRDahjzHYVr0G1-mwVwjqaFadzKpkVh3NqkO6-fKWPtadNe8X_1QmgJ-AmFb-yQa17cfg08f_Sf0LIJuEBw</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Gong, Jian</creator><creator>Shen, Xi-Hui</creator><creator>Qiu, Hui</creator><creator>Chen, Chao</creator><creator>Yang, Rong-Ge</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111201</creationdate><title>Rhesus Monkey TRIM5α Has Distinct HIV-1 Restriction Activity Among Different Mammalian Cell Lines</title><author>Gong, Jian ; Shen, Xi-Hui ; Qiu, Hui ; Chen, Chao ; Yang, Rong-Ge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-1ea3fd09b41cbd55bd93f653d02a91ab1706078f4d1e18d8a4e75d867de2f1623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Callithrix</topic><topic>Cloning, Molecular</topic><topic>Dogs</topic><topic>Genetic Variation</topic><topic>HeLa Cells</topic><topic>HIV Infections - immunology</topic><topic>HIV Infections - virology</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - immunology</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Macaca mulatta</topic><topic>Microbiology</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Fluorescence</topic><topic>Molecular Sequence Data</topic><topic>Proteins - genetics</topic><topic>Proteins - immunology</topic><topic>Species Specificity</topic><topic>Transduction, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gong, Jian</creatorcontrib><creatorcontrib>Shen, Xi-Hui</creatorcontrib><creatorcontrib>Qiu, Hui</creatorcontrib><creatorcontrib>Chen, Chao</creatorcontrib><creatorcontrib>Yang, Rong-Ge</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Jian</au><au>Shen, Xi-Hui</au><au>Qiu, Hui</au><au>Chen, Chao</au><au>Yang, Rong-Ge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rhesus Monkey TRIM5α Has Distinct HIV-1 Restriction Activity Among Different Mammalian Cell Lines</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>63</volume><issue>6</issue><spage>531</spage><epage>537</epage><pages>531-537</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>Rhesus monkey TRIM5α (TRIM5α
rh
), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to some cellular cofactors. Here we reported that TRIM5α
rh
can significantly reduce the infection efficiency of VSV-G pseudotyped HIV-1/MA-YFP virus in human epithelial carcinoma (HeLa) cells, moderately reduce in porcine kidney (PK-15) cells and have no effect on the pseudotyped virus infection in Madin-Darby canine kidney (MDCK) cells. Furthermore, we found that the different HIV-1 restriction activities have no relation with the intracellular localization of TRIM5α
rh
. These results indicate that the cellular environment is very important for the efficient anti-HIV-1 activity of TRIM5α
rh
. We speculate that some unknown factors required for HIV-1 infection inhibition activity are adequately expressed in HeLa cells, inadequately expressed in PK-15 cells and absent in MDCK cells.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>21947260</pmid><doi>10.1007/s00284-011-0009-z</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Biomedical and Life Sciences Biotechnology Callithrix Cloning, Molecular Dogs Genetic Variation HeLa Cells HIV Infections - immunology HIV Infections - virology HIV-1 - genetics HIV-1 - immunology Humans Life Sciences Macaca mulatta Microbiology Microscopy, Confocal Microscopy, Fluorescence Molecular Sequence Data Proteins - genetics Proteins - immunology Species Specificity Transduction, Genetic |
| title | Rhesus Monkey TRIM5α Has Distinct HIV-1 Restriction Activity Among Different Mammalian Cell Lines |
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