Technical Pearls for Swine Lung Transplantation

Background Since the advent of ex vivo lung perfusion (EVLP), there has been increased focus on swine models of lung transplantation; however, the anatomic differences between human and swine lungs and the technical challenges in performing porcine lung transplantation are not well described in the...

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Bibliographic Details
Published in:The Journal of surgical research 2011-11, Vol.171 (1), p.e107-e111
Main Authors: Karimi, Ashkan, M.D, Cobb, Jessica A., Ph.D, Staples, Edward D., M.D, Baz, Maher A., M.D, Beaver, Thomas M., M.D., M.P.H
Format: Article
Language:English
Subjects:
animal model
Animals
ex vivo lung perfusion
Humans
Lung - anatomy & histology
Lung - surgery
lung transplantation
Lung Transplantation - methods
Models, Animal
Species Specificity
Surgery
Swine - anatomy & histology
Tissue and Organ Harvesting - methods
Transplantation Immunology
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Summary:Background Since the advent of ex vivo lung perfusion (EVLP), there has been increased focus on swine models of lung transplantation; however, the anatomic differences between human and swine lungs and the technical challenges in performing porcine lung transplantation are not well described in the surgical literature. Methods Surgically important anatomic variations are described, and the technical measures taken to address them during harvest and transplantation are introduced. Results There are three surgically important anatomic variations in pigs. First, the right cranial lobe bronchus arises directly from the trachea, which makes right lung transplantation technically challenging if not prohibitive. Second, the left hemi-azygos vein is fully developed and courses upward through the posterior mediastinum, where it crosses the left pulmonary hilum and drains directly into the coronary sinus. During transplantation, this vein is ligated and dissected away to expose the underlying left pulmonary hilar structures. Third, the right inferior pulmonary vein crosses the midline to drain into the left atrium immediately adjacent to the left inferior pulmonary vein. During donor lung preparation, the right inferior pulmonary vein is ligated distally from the left atrium, which leaves an adequate atrial cuff around the left sided pulmonary veins for later anastomosis. Conclusion Experimental porcine lung transplantation is technically demanding. We have found recognition of the above described anatomical differences and technical nuances facilitate transplantation and provide reproducible results.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2011.05.067