High‐sensitivity capillary and microchip electrophoresis using electrokinetic supercharging

Electrokinetic supercharging (EKS) is considered as one of the most powerful online preconcentration techniques in electrophoresis. It combines the efficient preconcentration power of field‐amplified sample injection and the exceptional selective nature of transient isotachophoresis. It has a wide r...

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Bibliographic Details
Published in:Journal of separation science 2011-10, Vol.34 (20), p.2790-2799
Main Authors: Dawod, Mohamed, Chung, Doo Soo
Format: Article
Language:English
Subjects:
Animals
Capillarity
Deoxyribonucleic acid
DNA
DNA - analysis
DNA - isolation & purification
Electrokinetic supercharging
Electrokinetics
Electrophoresis
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Fragments
Humans
ions
Isotachophoresis
Isotachophoresis - instrumentation
Isotachophoresis - methods
Kinetics
Lab-On-A-Chip Devices
Microchip Analytical Procedures - methods
new methods
new technology
On-line systems
Online
Preconcentration
Proteins
Proteins - analysis
Proteins - isolation & purification
Sensitivity and Specificity
Stacking
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Summary:Electrokinetic supercharging (EKS) is considered as one of the most powerful online preconcentration techniques in electrophoresis. It combines the efficient preconcentration power of field‐amplified sample injection and the exceptional selective nature of transient isotachophoresis. It has a wide range of applications to different types of analytes ranging from small ions to large proteins and DNA fragments. This comprehensive review — up to date — provides listing for all the works, developments, and advances in EKS. The review will pay particular attention to innovations, new methodologies for manipulation, challenges for improving the detection sensitivity, and various applications of EKS in capillaries and microchips.
ISSN:1615-9306
1615-9314
1615-9314
DOI:10.1002/jssc.201100384