Phytochemical study of Uncaria guianensis leaves and antibacterial activity evaluatio

A chlorin compound, pheophorbide a ethyl ester and a mixture of sterols known as beta -sitosterol and stigmasterol, were isolated from the petroleum ether extract of Uncaria guianensis (Rubiaceae) leaves. Their structures were elucidated by detailed analysis of NMR spectra, including bidimensional t...

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Bibliographic Details
Published in:Acta amazonica 2011-06, Vol.41 (2), p.303-310
Main Authors: Prieto Rodriguez, JA, Patino Ladin, OJ, Lesmes, L, Lozano, J M, Cuca Suarez, LE
Format: Article
Language:Portuguese
Subjects:
Antibacterial activity
Escherichia coli
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Summary:A chlorin compound, pheophorbide a ethyl ester and a mixture of sterols known as beta -sitosterol and stigmasterol, were isolated from the petroleum ether extract of Uncaria guianensis (Rubiaceae) leaves. Their structures were elucidated by detailed analysis of NMR spectra, including bidimensional techniques and by comparison with literature data. The antibacterial activity for the pheophorbide a ethyl ester was evaluated against two Gram (+) strains: S. aureus ATCC 6538 y E. faecalis ATCC 29212 and three Gram (-) strains: E. coli ATCC 25922, S. typhimurium ATCC 14028s y S. typhimurium MS79535. aureus ATCC 6538 and E. fecalis ATCC 29212, finding significant activity against S. aureus 6538, E faecalis 29212, S. tiphymurium MS7953 and E. coli 25922.Original Abstract: Del extracto de eter de petroleo de hojas de Uncaria guianensis (Rubiaceae), se aislo un compuesto tipo clorina denominado ester etilico de feoforbida a y una mezcla de esteroles conocidos como beta -sitosterol y estigmasterol. Sus estructuras fueron elucidadas por analisis detallado de RMN, incluyendo tecnicas bidimensionales, y por comparacion con datos reportados en la literatura. Posteriormente, se evaluo la actividad antibacteriana al ester etilico de feoforbida a contra dos cepas Gram (+): S. aureus ATCC 6538 y E. faecalis ATCC 29212 y contra tres cepas Gram (-): E. coli ATCC 25922, S. typhimurium ATCC 14028s y S. typhimurium MS7953. Se encontro actividad significativa contra S. aureus, E.faecalis, E. coliy S. tiphymurium MS7953.
ISSN:0044-5967