Co-transcriptional architecture in a Y loop in Drosophila melanogaster

The Y loops of Drosophila spermatocytes are formed by the expression of huge individual transcription units on the Y chromosome and their large size provides a unique system for the investigation of the organisation of transcription in intact nuclei. By labelling ribonucleo-protein (RNP) components,...

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Bibliographic Details
Published in:Chromosoma 2011-08, Vol.120 (4), p.399-407
Main Authors: Redhouse, Juliet L., Mozziconacci, Julien, White, Robert A. H.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Biochemistry
Biomedical and Life Sciences
Cell Biology
Cell Nucleus - genetics
Chromatin - chemistry
Chromatin - genetics
Chromatin - metabolism
Chromosomes, Insect - chemistry
Chromosomes, Insect - genetics
Chromosomes, Insect - metabolism
Developmental Biology
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila Proteins - chemistry
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Eukaryotic Microbiology
Fertility - genetics
Human Genetics
In Situ Hybridization, Fluorescence
Life Sciences
Male
Microscopy, Confocal
Research Article
Ribonucleoproteins - chemistry
Ribonucleoproteins - genetics
Ribonucleoproteins - metabolism
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
RNA-Binding Proteins - chemistry
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Spermatocytes - cytology
Spermatocytes - metabolism
Transcription, Genetic
Y Chromosome - chemistry
Y Chromosome - genetics
Y Chromosome - metabolism
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Summary:The Y loops of Drosophila spermatocytes are formed by the expression of huge individual transcription units on the Y chromosome and their large size provides a unique system for the investigation of the organisation of transcription in intact nuclei. By labelling ribonucleo-protein (RNP) components, the loop chromatin and nascent transcripts in Y loop C, we reveal a highly structured organisation of RNP domains associated with nascent transcripts. We distinguish two types of RNP domain, a proximal domain that runs alongside the chromatin of loop C and a distal RNP domain that wraps around the proximal domain and the loop chromatin. The proximal domain is marked by the Pasilla protein, and separate distal subdomains are marked by the S5 antigen and Boule. We discuss the implications of this highly structured co-transcriptional architecture for the organisation of the process of transcription.
ISSN:0009-5915
1432-0886
DOI:10.1007/s00412-011-0321-1