Structure elucidation of the O-antigen of Salmonella enterica O51 and its structural and genetic relation to the O-antigen of Escherichia coli O23

The O-polysaccharide (O-antigen) of Salmonella enterica O51 was isolated by mild acid degradation of the lipopolysaccharide and its structure was established using sugar analysis and NMR spectroscopy. The O-antigen of Escherichia coli O23, whose structure was elucidated earlier, possesses a similar...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2011-07, Vol.76 (7), p.774-779
Main Authors: Perepelov, A. V., Liu, Bin, Guo, Dan, Senchenkova, S. N., Shahskov, A. S., Feng, Lu, Wang, Lei, Knirel, Y. A.
Format: Article
Language:English
Subjects:
Antigens
Bacterial genetics
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Carbohydrate Sequence
Chemicals
E coli
Escherichia coli
Escherichia coli - chemistry
Escherichia coli - genetics
Escherichia coli - immunology
Genes
Genetic aspects
Genetics
Introduced species
Life Sciences
Microbial polysaccharides
Microbiology
Mitogens
Molecular Sequence Data
Multigene Family
Nuclear magnetic resonance spectroscopy
Nuclear Magnetic Resonance, Biomolecular
O Antigens - chemistry
O Antigens - genetics
O Antigens - immunology
Salmonella
Salmonella enterica
Salmonella enterica - chemistry
Salmonella enterica - genetics
Salmonella enterica - immunology
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Summary:The O-polysaccharide (O-antigen) of Salmonella enterica O51 was isolated by mild acid degradation of the lipopolysaccharide and its structure was established using sugar analysis and NMR spectroscopy. The O-antigen of Escherichia coli O23, whose structure was elucidated earlier, possesses a similar structure and differs only in the presence of an additional lateral α-D-Glc p residue at position 6 of the GlcNAc residue in the main chain. Sequencing of the O-antigen gene clusters of S. enterica O51 and E. coli O23 revealed the same genes with a high-level similarity. By comparison with opened gene databases, all genes expected for the synthesis of the common structure of the two O-antigens were assigned functions. It is suggested that the gene clusters of both bacteria originated from a common ancestor, whereas the O-antigen modification in E. coli O23, which, most probably, is induced by prophage genes outside the gene cluster, could be introduced after the species divergence.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297911070078