Ultrasensitive immunosensing of tuberculosis CFP-10 based on SPR spectroscopy

• Published in: Sensors and actuators. B, Chemical Chemical, 2011-08, Vol.156 (1), p.271-275
• Main Authors: Hong, Seong Cheol, Chen, Hongxia, Lee, Jaewook, Park, Hye-Kyung, Kim, Young Sik, Shin, Hyun-Chul, Kim, Cheol-Min, Park, Tae Jung, Lee, Seok Jae, Koh, Kwangnak, Kim, Hwa-Jung, Chang, Chulhun L., Lee, Jaebeom
• Format: Article
• Language: English
• Subjects: antigens; Biosensor; CFP-10; Gold; immunosensors; monoclonal antibodies; Mycobacterium; patients; proteins; spectroscopy; surface plasmon resonance; Surface plasmon resonance (SPR) spectroscopy; tuberculosis; Tuberculosis (TB)
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2011.04.032

An antigen (Ag), CFP-10, found in tissue fluids of tuberculosis (TB) patients may be an ultimate candidate for use as a sensitive TB marker with a sensing method for early simplified diagnosis of TB. In this study, chemical and optical optimizations were carried out using novel immuno-materials for establishment of a self-assembled surface plasmon resonance (SPR) optical immunosensor system for detection of CFP-10, which is valuable for pre-clinical work, prior to conduct of massive clinical observations. For creation of a simple sensing interface, a monoclonal antibody (anti-CFP-10) was immobilized directly on a gold surface, followed by blocking with cystamine. Orientation and accessibility of anti-CFP-10 were assessed by the selective binding of CFP-10. Recent results indicate that the reusability of the sensor chip adopting the cystamine method was found to be preferable to other immobilization methods. A linear relationship was well correlated between SPR angle shift and CFP concentrations in the range from 100 ng mL −1 to 1 μg mL −1. Modification of the SPR chip with antibody provides a simple experimental platform for investigation of isolated proteins under experimental conditions resembling those of their native environment.