Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (Verasper variegatus)

In the present study, methodology of gynogenetic induction in spotted halibut were developed and optimized; the sex ratio of putative meiotic gynogenetic diploids was determined using AFLP-based molecular sexing technique; the homozygosity of gynogenetic population was assessed as opposed to cultiva...

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Bibliographic Details
Published in:Aquaculture international 2010-10, Vol.18 (6), p.1151-1161
Main Authors: Ji, Xiang-Shan, Chen, Song-Lin, Yang, Jing-Feng, Ma, Hong-Yu, Jiang, Yun-Liang
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Chemical analysis
Eggs
Females
Fisheries
Freshwater & Marine Ecology
Genetic engineering
Gynogenesis
Homozygosity
Larvae
Life Sciences
Marine
Seawater
Sex ratio
Sperm
Verasper variegatus
Water analysis
Zoology
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Summary:In the present study, methodology of gynogenetic induction in spotted halibut were developed and optimized; the sex ratio of putative meiotic gynogenetic diploids was determined using AFLP-based molecular sexing technique; the homozygosity of gynogenetic population was assessed as opposed to cultivated population. The results showed that high percentage of meiotic gynogenetic diploids were generated when the eggs fertilized with irradiated heterologous sea perch frozen sperm (30-50 mJ cm⁻²) were cold shocked in sea water of −1°C for 40-75 min at 5 min after fertilization. About 15,200 diploid gynogenetic larvae were achieved and they exhibited normal morphology similar to diploid control. The gynogenetic diploids were 100% female, which first confirmed the female homogamete (XX/XY) sex determination in spotted halibut. The genetic analysis showed that the average H O was, respectively, 0.404 and 0.724 in gynogenetic population and cultivated population, indicating an increase of homozygosity in gynogenetic population.
ISSN:0967-6120
1573-143X
DOI:10.1007/s10499-010-9330-y