improved nitrilase-mediated bioprocess for synthesis of nicotinic acid from 3-cyanopyridine with hyperinduced Nocardia globerula NHB-2

• Published in: Journal of industrial microbiology & biotechnology 2011-09, Vol.38 (9), p.1235-1243
• Main Authors: Sharma, Nitya Nand, Sharma, Monica, Bhalla, Tek Chand
• Format: Article
• Language: English
• Subjects: Acids; Aminohydrolases - metabolism; Ammonia; Biocatalysts; Biochemistry; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; bioprocessing; Biosynthesis; Biotechnology; Catalysis; Culture Media; Enzymes; Fundamental and applied biological sciences. Psychology; Genetic Engineering; Inorganic Chemistry; Life Sciences; Microbiology; niacin; Niacin - biosynthesis; nitrilase; nitriles; Nitriles - metabolism; Nocardia; Nocardia - enzymology; Nocardia globerula; Original Paper; Oxidation; Pyridines - metabolism; Studies
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-010-0902-7

Nitrilase of Nocardia globerula NHB-2 was induced by short-chain aliphatic nitriles (valeronitrile > isobutyronitrile > butyronitrile > propionitrile) and exhibited activity towards aromatic nitriles (benzonitrile > 3-cyanopyridine > 4-cyanopyridine > m-tolunitrile > p-tolunitrile). Hyperinduction of nitrilase (6.67 U mgDCW −1, 18.7 U mL−1) was achieved in short incubation time (30 h, 30°C) through multiple feeding of isobutyronitrile in the growth medium. The nitrilase of this organism exhibits both substrate and product inhibition effects. In a fed batch reaction at 1 L scale using hyperinduced resting cells corresponding to 10 U mL−1 nitrilase activity (1.5 mgDCW mL−1), a total of 123.11 g nicotinic acid was produced at a rate of 24 g h−1 gDCW −1.