Effects of pH, glucose and iron sulfate concentration on the yield of biohydrogen by Clostridium butyricum EB6

A local bacterial isolate from palm oil mill effluent (POME) sludge, identified as Clostridium butyricum EB6, was used for biohydrogen production. Optimization of biohydrogen production was performed via statistical analysis, namely response surface methodology (RSM), with respect to pH, glucose and...

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Bibliographic Details
Published in:International journal of hydrogen energy 2009-11, Vol.34 (21), p.8859-8865
Main Authors: Chong, Mei-Ling, Abdul Rahman, Nor'Aini, Yee, Phang Lai, Aziz, Suraini Abd, Rahim, Raha Abdul, Shirai, Yoshihito, Hassan, Mohd Ali
Format: Article
Language:English
Subjects:
Alternative fuels. Production and utilization
Applied sciences
Biohydrogen
Clostridium butyricum
Clostridium butyricum EB6
Energy
Exact sciences and technology
Fuels
Hydrogen
Microbial
Optimization
Response surface methodology
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Summary:A local bacterial isolate from palm oil mill effluent (POME) sludge, identified as Clostridium butyricum EB6, was used for biohydrogen production. Optimization of biohydrogen production was performed via statistical analysis, namely response surface methodology (RSM), with respect to pH, glucose and iron concentration. The results show that pH, glucose concentration and iron concentration significantly influenced the biohydrogen gas production individually, interactively and quadratically ( P < 0.05). The center composite design (CCD) results indicated that pH 5.6, 15.7 g/L glucose and 0.39 g/L FeSO 4 were the optimal conditions for biohydrogen production, yielding 2.2 mol H 2/mol glucose. In confirmation of the experimental model, t-test results showed that curve fitted to the experimental data had a high confidence level, at 95% with t = 2.225. Based on the results of this study, optimization of the culture conditions for C. butyricum EB6 significantly increased the production of biohydrogen.
ISSN:0360-3199
1879-3487
DOI:10.1016/j.ijhydene.2009.08.061