Cryopreservation and transplantation of sexually immature gonads of rainbow trout

The objective of this investigation was to cryopreserve sexually immature gonads of rainbow trout (RBT). Gonads from juvenile male or female RBT were cryopreserved and stored in liquid nitrogen. Individual testes were thawed and surgically transplanted to isogenic male trout; individual ovaries were...

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Bibliographic Details
Published in:Fish physiology and biochemistry 2003-01, Vol.28 (1-4), p.459-462
Main Author: Cloud, J.G.
Format: Article
Language:English
Subjects:
Aquaculture
Fertility
Fish
Gonads
In vitro fertilization
Nitrogen
Oncorhynchus mykiss
Salmon
Salmonidae
Trout
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Summary:The objective of this investigation was to cryopreserve sexually immature gonads of rainbow trout (RBT). Gonads from juvenile male or female RBT were cryopreserved and stored in liquid nitrogen. Individual testes were thawed and surgically transplanted to isogenic male trout; individual ovaries were autografted. Four weeks following transplantation, all male fish were injected with salmon pituitary extract for 8-10 weeks. The transplanted testis was present in 5 of 8 animals in replicate 1 and in 3 of 6 animals in replicate 2. Fertility of sperm obtained from individual testes in replicate 2 was evaluated by in vitro fertilization. Sperm from the intact control testes had a fertilization rate of 95±3% (mean ± SEM), and sperm from transplanted testes had a fertilization rate of 78±7%. Ovaries that were frozen in liquid nitrogen, thawed and autografted to an ectopic site were not present at five months following surgery.[PUBLICATION ABSTRACT]
ISSN:0920-1742
1573-5168
DOI:10.1023/B:FISH.0000030629.13860.37