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Selection of biochemical mutants of Aspergillus niger with enhanced catalase production
The production of extracellular catalase in a submerged culture by a number of biochemical mutants has been evaluated. Eight of these mutants showed increased extracellular catalase, the level of which ranged widely in individual cases from 44% to over 94% in comparison with the parental strain. Stu...
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Published in: | Applied microbiology and biotechnology 1997-03, Vol.47 (3), p.313-316 |
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description | The production of extracellular catalase in a submerged culture by a number of biochemical mutants has been evaluated. Eight of these mutants showed increased extracellular catalase, the level of which ranged widely in individual cases from 44% to over 94% in comparison with the parental strain. Studies of the relationship between a criterion of selection and the frequency of mutation showed that the highest frequency of positive mutations (15.8% and 24.2%) was obtained with respect to mutants resistant to ethidium bromide (1 mmol/l) and sodium gluconate (45%) respectively. The time course of growth and enzyme production by the most active mutant, AM-20, showed extra- and intracellular catalase activities increasing about 2- and 2.6- fold respectively, compared with the parental strain. |
doi_str_mv | 10.1007/s002530050933 |
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Eight of these mutants showed increased extracellular catalase, the level of which ranged widely in individual cases from 44% to over 94% in comparison with the parental strain. Studies of the relationship between a criterion of selection and the frequency of mutation showed that the highest frequency of positive mutations (15.8% and 24.2%) was obtained with respect to mutants resistant to ethidium bromide (1 mmol/l) and sodium gluconate (45%) respectively. 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Eight of these mutants showed increased extracellular catalase, the level of which ranged widely in individual cases from 44% to over 94% in comparison with the parental strain. Studies of the relationship between a criterion of selection and the frequency of mutation showed that the highest frequency of positive mutations (15.8% and 24.2%) was obtained with respect to mutants resistant to ethidium bromide (1 mmol/l) and sodium gluconate (45%) respectively. The time course of growth and enzyme production by the most active mutant, AM-20, showed extra- and intracellular catalase activities increasing about 2- and 2.6- fold respectively, compared with the parental strain.</description><subject>Analysis</subject><subject>Aspergillus</subject><subject>Aspergillus niger</subject><subject>Aspergillus niger - enzymology</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Biological Sciences</subject><subject>Biotechnology</subject><subject>Catalase - biosynthesis</subject><subject>Enzymes</subject><subject>Eukaryotes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic technics</subject><subject>Glucose Oxidase - metabolism</subject><subject>Methods. Procedures. 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Psychology</topic><topic>Genetic technics</topic><topic>Glucose Oxidase - metabolism</topic><topic>Methods. Procedures. 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Eight of these mutants showed increased extracellular catalase, the level of which ranged widely in individual cases from 44% to over 94% in comparison with the parental strain. Studies of the relationship between a criterion of selection and the frequency of mutation showed that the highest frequency of positive mutations (15.8% and 24.2%) was obtained with respect to mutants resistant to ethidium bromide (1 mmol/l) and sodium gluconate (45%) respectively. The time course of growth and enzyme production by the most active mutant, AM-20, showed extra- and intracellular catalase activities increasing about 2- and 2.6- fold respectively, compared with the parental strain.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>9114519</pmid><doi>10.1007/s002530050933</doi><tpages>4</tpages></addata></record> |
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subjects | Analysis Aspergillus Aspergillus niger Aspergillus niger - enzymology Bacteria Biological and medical sciences Biological Sciences Biotechnology Catalase - biosynthesis Enzymes Eukaryotes Fundamental and applied biological sciences. Psychology Genetic technics Glucose Oxidase - metabolism Methods. Procedures. Technologies Microbiological chemistry Microbiological synthesis Mutant screening Mutants Mutation Properties Synthesis |
title | Selection of biochemical mutants of Aspergillus niger with enhanced catalase production |
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