Mechanisms of waterborne Cu toxicity to the pond snail Lymnaea stagnalis: Physiology and Cu bioavailability

• Published in: Ecotoxicology and environmental safety 2011-09, Vol.74 (6), p.1471-1479
• Main Authors: Ng, Tania Y.-T., Pais, Nish M., Wood, Chris M.
• Format: Article
• Language: English
• Subjects: acute exposure; Animal and plant ecology; Animal, plant and microbial ecology; Animals; Applied ecology; Bioavailability; Biological and medical sciences; Biological Availability; Calcium - metabolism; chronic exposure; Copper; Copper - metabolism; Copper - toxicity; Detoxification; Ecotoxicology, biological effects of pollution; foods; Fresh Water - chemistry; Fresh water ecosystems; Freshwater; Fundamental and applied biological sciences. Psychology; General aspects; Granular materials; granules; Growth; Ionoregulation; lethal concentration 50; Lymnaea - drug effects; Lymnaea - growth & development; Lymnaea - metabolism; Lymnaea stagnalis; Metallothionein - metabolism; metals; mortality; Oxidative Stress; Physiology; Pond snail; Ponds; proteins; Snails; Sodium - metabolism; Synecology; Thiobarbituric Acid Reactive Substances - metabolism; thiobarbituric acid-reactive substances; tissue distribution; Toxicity; Toxicity Tests, Acute; Water Pollutants, Chemical - metabolism; Water Pollutants, Chemical - toxicity; Water quality; water quality criteria
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2011.03.010

We examined the mechanisms of toxicity of waterborne Cu to the freshwater pond snail Lymnaea stagnalis. The snail is one of the most sensitive species to acute Cu exposure (96 h LC 50, LC 20: 24.9, 18.0 μg l −1); they are not protected by the water quality criteria of the US EPA. Tissue Na and Ca were also reduced by Cu in the acute exposure. In contrast, during 28 d chronic exposures to Cu in the presence of food, which resulted in higher DOC concentrations, there was no significant mortality but an inhibition of growth, which may reflect a re-allocation of resources to detoxification. Cu detoxification was evidenced in chronic exposure by increases in metallothionein-like protein concentrations and Cu binding to metal-rich granules, decreases in thiobarbituric acid-reactive substances, and changes in the subcellular distribution in the soft tissues. Our results demonstrated that apart from external Cu bioavailability, compartmentalization of metals within the cells can alter toxicity of Cu to the snails.