In vitro assessment of cellular responses to rod-shaped hydroxyapatite nanoparticles of varying lengths and surface areas

Abstract Rod-shaped hydroxyapatite nanoparticles of varying dimensions ( 60 ± 10, 120 ± 15, 240 ± 30 nm in length, labeled respectively as nHA60, nHA120 and nHA240) with specific surface areas (47.02, 23.33, 46.12 nm2, respectively), were synthesized and their effects on cell viability, reactive oxy...

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Bibliographic Details
Published in:Nanotoxicology 2011-06, Vol.5 (2), p.182-194
Main Authors: Zhao, Xinxin, Heng, Boon Chin, Xiong, Sijing, Guo, Jun, Tan, Timothy Thatt-Yang, Boey, Freddy Yin Chiang, Ng, Kee Woei, Loo, Joachim Say Chye
Format: Article
Language:English
Subjects:
Alizarin Red-S
Animals
Anthraquinones - metabolism
biocompatibility
Cell Line
Coloring Agents - metabolism
Durapatite - chemistry
Durapatite - metabolism
Humans
Hydroxyapatite
length effect
Materials Testing
Mice
Nanoparticles - chemistry
Particle Size
particle/cell interaction
reactive oxygen species
rod
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction
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Summary:Abstract Rod-shaped hydroxyapatite nanoparticles of varying dimensions ( 60 ± 10, 120 ± 15, 240 ± 30 nm in length, labeled respectively as nHA60, nHA120 and nHA240) with specific surface areas (47.02, 23.33, 46.12 nm2, respectively), were synthesized and their effects on cell viability, reactive oxygen species generation and cellular interaction with BEAS-2B, RAW264.7 and HepG2 were investigated. In vitro exposure of these cell lines to rod shape nHA particles within a range of 10-300 μg/ml for 24 h did not significantly alter cell viability studied by the WST-8 assay. A significant increase in reactive oxygen species (ROS) generation was however observed with the dihydrofluorescein diacetate (DFDA) assay after 4 h incubation with these nanoparticles. The lowest level of ROS generation was observed with nHA120 (with the smallest specific surface area); whereas nHA60 and nHA240 exhibited comparable ROS generation. Subsequently, the Alizarin Red-S (ARS) assay indicated a weaker association of calcium with cells compared to nHA60 and nHA240. The results thus suggest that high surface area may increase cell-particle interaction, which in turn influenced ROS generation. The combined results from all the cell lines thus indicated high biocompatibility of rod-shaped nHA.
ISSN:1743-5390
1743-5404
DOI:10.3109/17435390.2010.503943