Masu salmon (Oncorhynchus masou) zona pellucida protein B: cDNA cloning and tissue expression

A full-length cDNA clone encoding zona pellucida protein B (ZPB) was isolated for the ovary of masu salmon. The deduced amino acid sequence of ZPB contained two domains commonly observed in ZPB of both mammals and teleosts. Using gene-specific primers for ZPB in masu salmon, RT-PCR analyses revealed...

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Bibliographic Details
Published in:Fish physiology and biochemistry 2003-01, Vol.28 (1-4), p.333-334
Main Authors: Fujita, T, Fukada, H, Hara, A
Format: Article
Language:English
Subjects:
Amino acids
Cloning
Fish
Genetic engineering
Oncorhynchus masou
Salmon
Teleostei
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Summary:A full-length cDNA clone encoding zona pellucida protein B (ZPB) was isolated for the ovary of masu salmon. The deduced amino acid sequence of ZPB contained two domains commonly observed in ZPB of both mammals and teleosts. Using gene-specific primers for ZPB in masu salmon, RT-PCR analyses revealed that expression of ZPB was isolated to the ovary. These results suggest involvement of an ovary-derived zona pellucida protein in formation of the chorion in masu salmon.
ISSN:0920-1742
1573-5168
DOI:10.1023/B:FISH.0000030575.78617.34