in vivo genotoxicity of cisplatin, isoflurane and halothane evaluated by alkaline comet assay in Swiss albino mice

The aim of this study was to evaluate the genotoxicity of repeated exposure to isoflurane or halothane and compare it with the genotoxicity of repeated exposure to cisplatin. We also determined the genotoxicity of combined treatment with inhalation anaesthetics and cisplatin on peripheral blood leuc...

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Bibliographic Details
Published in:Journal of applied genetics 2011-08, Vol.52 (3), p.355-361
Main Authors: Brozovic, Gordana, Orsolic, Nada, Knezevic, Fabijan, Horvat Knezevic, Anica, Benkovic, Vesna, Sakic, Katarina, Borojevic, Nikola, Dikic, Domagoj
Format: Article
Language:English
Subjects:
albino
Anesthetics
Animal Genetics and Genomics
Animal Genetics ∙ Original Paper
Antimitotic agents
Antineoplastic agents
Biomedical and Life Sciences
Brain
Cisplatin
Comet assay
DNA damage
Drugs
Ethylenediaminetetraacetic acid
Genotoxicity
Halides
Halothane
Hepatocytes
Human Genetics
Inhalation
Isoflurane
Kidney
kidney cells
Leukocytes
Life Sciences
Liver
Liver diseases
mice
Microbial Genetics and Genomics
Peripheral blood
Plant Genetics and Genomics
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Summary:The aim of this study was to evaluate the genotoxicity of repeated exposure to isoflurane or halothane and compare it with the genotoxicity of repeated exposure to cisplatin. We also determined the genotoxicity of combined treatment with inhalation anaesthetics and cisplatin on peripheral blood leucocytes (PBL), brain, liver and kidney cells of mice. The mice were divided into six groups as follows: control, cisplatin, isoflurane, cisplatin–isoflurane, halothane and cisplatin–halothane, and were exposed respectively for three consecutive days. The mice were treated with cisplatin or exposed to inhalation anaesthetic; the combined groups were exposed to inhalation anaesthetic after treatment with cisplatin. The alkaline comet assay was performed. All drugs had a strong genotoxicity (P 
ISSN:1234-1983
2190-3883
DOI:10.1007/s13353-011-0046-0