Quantitative PCR assay for the detection of the parasitic ciliate Cryptocaryon irritans

We developed a quantitative PCR assay for detecting the parasitic ciliate Cryptocaryon irritans , which causes “white spot disease” in marine fishes, from the natural environment. A specific primer set for C . irritans was designed and its high specificity was confirmed in silico: almost all of the...

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Bibliographic Details
Published in:Fisheries science 2011-07, Vol.77 (4), p.607-613
Main Authors: Taniguchi, Akito, Onishi, Hiroyuki, Eguchi, Mitsuru
Format: Article
Language:English
Subjects:
Aquaculture
Aquariums
Bioassays
Biomedical and Life Sciences
Chemical analysis
Cryptocaryon irritans
Design
Fish & Wildlife Biology & Management
Fish diseases
Fisheries
Fisheries management
Food Science
Freshwater & Marine Ecology
Genes
Genomes
Life Sciences
Marine
Marine fish
Natural environment
Original Article
Parasites
Protozoa
Seawater
Studies
Water analysis
Water depth
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Summary:We developed a quantitative PCR assay for detecting the parasitic ciliate Cryptocaryon irritans , which causes “white spot disease” in marine fishes, from the natural environment. A specific primer set for C . irritans was designed and its high specificity was confirmed in silico: almost all of the sequences deposited in the GenBank nucleotide database were covered, 22/23 for the forward primer and 7/7 for the reverse primer. We estimated that there were 3,415.9 rRNA gene copies per genome of C . irritans . In artificial mixture experiments to validate whether the qPCR assay is applicable to natural samples, the estimated copy numbers showed significantly positive correlations with the number of theronts added ( p  
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-011-0362-7