Bioconversion of nitriles by Candida guilliermondii CCT 7207 cells immobilized in barium alginate

• Published in: Applied microbiology and biotechnology 2001-09, Vol.56 (5/6), p.757-761
• Main Authors: Dias, J.C.T, Rezende, R.P, Linardi, V.R
• Format: Article
• Language: English
• Subjects: Acetic acid; Alginates; Amides; Barium; barium alginate; Barium Compounds; Bioconversions. Hemisynthesis; Biodegradation, Environmental; Biological and medical sciences; Bioremediation; Biotechnology; biotransformation; Candida - growth & development; Candida - metabolism; Candida - ultrastructure; Candida guilliermondii; cell adhesion; Cell adhesion & migration; Cells, Immobilized; Chlorides; Culture Media; Fundamental and applied biological sciences. Psychology; Glucuronic Acid; Hexuronic Acids; Immobilization of organelles and whole cells; Immobilization techniques; immobilized cells; Methods. Procedures. Technologies; Meyerozyma guilliermondii; Microscopy, Electron; nitriles; Nitriles - metabolism; nitrogen; Nitrogen sources; Organic acids; Scanning electron microscopy; Yeasts
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s002530100681

Nitrile degradation by Candida guilliermondii CCT 7207 using free and immobilized cell systems was compared. Different specific growth rates were observed for immobilized (mu(max)=0.021 h(-1)) and the free cells (mu(max)=0.029 h(-1)). The maximum specific rate of acetic acid formation was 0.387 h(-1) and 0.266 h(-1) for free and immobilized cells, respectively. Cell adhesion to the support materials was confirmed by scanning electron microscopy. When immobilized, the yeast was able to use high nitrile and amide concentrations (aliphatic and aromatic) as nitrogen sources. The results suggest that C. guilliermondii CCT 7207 presents a physiological pattern potentially useful for the bioremediation of polluted environments or for the bioproduction of amides and organic acid of high commercial value.