Identification and sequence analysis of the Condylorrhiza vestigialis MNPV p74 gene

The baculovirus Condylorrhiza vestigialis multiple nucleopolyhedrovirus (CoveMNPV), isolated from C. vestigialis infected larvae in Paraná (Brazil), was identified in our laboratory. A full-length clone was obtained from the CoveMNPV genome, of the gene that encodes the homolog to baculoviral p74, e...

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Bibliographic Details
Published in:Virus genes 2011-12, Vol.43 (3), p.471-475
Main Authors: Castro, Maria Elita B, Paula, Débora P, Almeida, Geraldo F, Ribeiro, Zilda Maria A, Souza, Marlinda L, Inglis, Peter W, Ribeiro, Bergmann M
Format: Article
Language:English
Subjects:
Animals
Baculovirus
Biomedical and Life Sciences
Biomedicine
Choristoneura fumiferana
genes
larvae
Lepidoptera
Medical Microbiology
Molecular Sequence Data
Moths - virology
Nuclear polyhedrosis virus
Nucleopolyhedrovirus
Nucleopolyhedrovirus - classification
Nucleopolyhedrovirus - genetics
Nucleopolyhedrovirus - isolation & purification
open reading frames
pathogenicity
Phylogeny
Plant Sciences
sequence analysis
Viral Envelope Proteins - genetics
Viral Envelope Proteins - metabolism
Virology
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Summary:The baculovirus Condylorrhiza vestigialis multiple nucleopolyhedrovirus (CoveMNPV), isolated from C. vestigialis infected larvae in Paraná (Brazil), was identified in our laboratory. A full-length clone was obtained from the CoveMNPV genome, of the gene that encodes the homolog to baculoviral p74, essential for oral infectivity which was then sequenced and characterized. The CoveMNPV p74 gene (GenBank accession number EU919397) contains an ORF of 1935 bp that encodes a deduced protein of 73.61 kDa. The phylogenetic affiliations of the CoveMNPV gene were determined by a heuristic search of 40 aligned baculovirus p74 nucleotide sequences using maximum parsimony (PAUP 4.0b4a). The phylogenetic analysis placed CoveMNPV within lepidopteran nucleopolyhedrovirus (NPV) Group I, Clade A, as being the closest to Choristoneura fumiferana defective NPV.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-011-0640-6