Multimerized siRNA Cross-linked by Gold Nanoparticles

In this study, siRNAs terminated with thiol groups were multimerized and cross-linked using ∼5 nm gold nanoparticles (AuNPs) via Au–S chemisorption that can be intracellularly reduced. AuNPs immobilized with single-stranded antisense siRNA were assembled with those with single-stranded sense siRNA v...

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Bibliographic Details
Published in:Bioconjugate chemistry 2011-10, Vol.22 (10), p.1962-1969
Main Authors: Kong, Won Ho, Bae, Ki Hyun, Hong, Cheol Am, Lee, Yuhan, Hahn, Sei Kwang, Park, Tae Gwan
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Cells
Chemical compounds
Cross-Linking Reagents - chemistry
Enzymes
Gene Silencing
Gold
Gold - chemistry
Green Fluorescent Proteins - genetics
Humans
Nanoparticles
Nanoparticles - chemistry
Ribonucleic acid
RNA
RNA, Small Interfering - administration & dosage
RNA, Small Interfering - chemistry
RNA, Small Interfering - genetics
Sulfhydryl Compounds - chemistry
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Summary:In this study, siRNAs terminated with thiol groups were multimerized and cross-linked using ∼5 nm gold nanoparticles (AuNPs) via Au–S chemisorption that can be intracellularly reduced. AuNPs immobilized with single-stranded antisense siRNA were assembled with those with single-stranded sense siRNA via complementary hybridization or assembled with those with single-stranded dimeric sense siRNA. The multimerized siRNA cross-linked by AuNPs showed increased charge density and enhanced enzymatic stability, and exhibited good complexation behaviors with a polycationic carrier, linear polyethylenimine (L-PEI). The resultant multi-siRNA/AuNPs/L-PEI polyelectrolyte complexes exhibited far greater gene silencing efficiencies of green fluorescent protein (GFP) and vascular endothelial growth factor (VEGF) compared to naked siRNA complexes. They could also be visualized by micro-CT imaging. The results suggest that AuNP-mediated multimerization of siRNAs could be a rational approach to achieve both gene silencing and imaging at a target tissue simultaneously.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc200172p