Metabolomic profiling of the autosomal dominant polycystic kidney disease rat model

Background Autosomal dominant polycystic kidney disease (ADPKD) is an inherited systemic disease characterized by renal cyst expansion, resulting in renal failure. With the progression of renal damage, the accumulation of uremic compounds is recently reported to subsequently cause further renal dama...

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Bibliographic Details
Published in:Clinical and experimental nephrology 2011-10, Vol.15 (5), p.676-687
Main Authors: Toyohara, Takafumi, Suzuki, Takehiro, Akiyama, Yasutoshi, Yoshihara, Daisuke, Takeuchi, Yoichi, Mishima, Eikan, Kikuchi, Koichi, Suzuki, Chitose, Tanemoto, Masayuki, Ito, Sadayoshi, Nagao, Shizuko, Soga, Tomoyoshi, Abe, Takaaki
Format: Article
Language:English
Subjects:
Aconitic Acid - blood
Animals
Disease Models, Animal
Electrophoresis, Capillary
Hippurates - blood
Humans
Indican - blood
Male
Mass Spectrometry
Medicine
Medicine & Public Health
Metabolomics
Nephrology
Original Article
Polycystic Kidney, Autosomal Dominant - blood
Polycystic Kidney, Autosomal Dominant - physiopathology
Rats
Renal Insufficiency - blood
Urology
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Summary:Background Autosomal dominant polycystic kidney disease (ADPKD) is an inherited systemic disease characterized by renal cyst expansion, resulting in renal failure. With the progression of renal damage, the accumulation of uremic compounds is recently reported to subsequently cause further renal damage and hypertension. Finding uremic toxins and sensitive markers for detecting the early stage of ADPKD is necessary to clarify its pathophysiological process and to prevent its progression. The aim of this study was to analyze the profile of uremic retention solutes of ADPKD by capillary electrophoresis–mass spectrometry (CE-MS) using the Han:SPRD rat model. Methods Two hundred and ninety-seven cations and 190 anions were comprehensively analyzed by CE-MS in Han:SPRD rats and control rats. Results We found 21 cations and 19 anions that accumulated significantly in the heterozygous (Cy/+) ADPKD rat model compared with control rats. Among the compounds, increases in 5-methyl-2′-deoxycytidine, glucosamine, ectoine, allantoate, α-hydroxybenzoate, phenaceturate and 3-phenylpropionate and decreases in 2-deoxycytidine, decanoate and 10-hydroxydecanoate were newly identified in the ADPKD Cy/+ rats. Conclusion We identified uremic retention solutes in ADPKD Cy/+ rats. Compounds related to ADPKD could be useful markers for detecting the early stage of ADPKD.
ISSN:1342-1751
1437-7799
DOI:10.1007/s10157-011-0467-4