Secretory IgA-Fcα receptor interaction modulating phagocytosis and microbicidal activity by phagocytes in human colostrum of diabetics

• Published in: APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2011-10, Vol.119 (10), p.710-719
• Main Authors: FRANçA, EDUARDO LUZíA, MORCELI, GLILCIANE, FAGUNDES, DANNY LAURA GOMES, RUDGE, MARILZA VIEIRA CUNHA, CALDERON, IRACEMA DE MATTOS PARANHOS, HONORIO-FRANçA, ADENILDA CRISTINA
• Format: Article
• Language: English
• Subjects: Adolescent; Biological and medical sciences; colostrum; Colostrum - cytology; Colostrum - immunology; Colostrum - microbiology; Cross-Sectional Studies; diabetes; Diabetes Mellitus - immunology; Diabetes Mellitus - microbiology; EPEC; Escherichia coli - immunology; Escherichia coli Infections - immunology; Escherichia coli Infections - microbiology; Escherichia coli Infections - prevention & control; FcαRI; Female; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Humans; Immunoglobulin A, Secretory - immunology; Immunoglobulin A, Secretory - pharmacology; Infant, Newborn; Infectious diseases; Macrophages - cytology; Macrophages - immunology; Medical sciences; Microbiology; Middle Aged; phagocytes; Phagocytosis - immunology; Pregnancy; Pregnancy in Diabetics - immunology; Receptors, Fc - immunology; SIgA; Young Adult
• ISSN: 0903-4641; 1600-0463
• DOI: 10.1111/j.1600-0463.2011.02789.x

França EL, Morceli G, Fagundes DLG, Rudge MVC, Calderon I de MP, Honorio‐França AC. Secretory IgA–Fcα receptor interaction modulating phagocytosis and microbicidal activity by phagocytes in human colostrum of diabetics. APMIS 2011; 119: 710–19. The effects of secretory immunoglobulin A (SIgA) interaction with its specific Fcα receptors on colostral phagocytes needs further investigation, especially with respect to diabetic women. Accordingly, we studied the colostrum of hyperglycemic women to assess SIgA interactions with Fcα receptors of macrophages as well as the functional activity of these cells. The women were divided for colostrum sampling according to their glycemic status: normoglycemia (N = 51), mild hyperglycemia (N = 23), and diabetes (N = 25) groups. We determined the FcαR expression, the IgA on the surface and the surface‐bound IgA in colostrum macrophages. We also evaluated the superoxide release and bactericidal killing of these cells. Colostral phagocytes expressed FcαR, contained IgA on the surface and are able to bind to purified SIgA. The bactericidal activity of colostral phagocytes from the hyperglycemic women was similar to that of normoglycemic only when SIgA was used as opsonin. Addition of a MoAb anti‐human Fcα receptor resulted in a significant decrease of superoxide release and bacterial killing by macrophages when bacteria were opsonized with purified SIgA, suggesting an interaction between SIgA and FcαR. The stimulatory effects of SIgA on the functional activity of phagocytes therefore protect infants, especially of diabetic women, against intestinal infections.