Epithelial-mesenchymal interactions in breast cancer: evidence for a role of nuclear localized β-catenin in carcinoma-associated fibroblasts

• Published in: Histopathology 2011-10, Vol.59 (4), p.609-618
• Main Authors: Verghese, Eldo T, Shenoy, Hrishikesh, Cookson, Victoria J, Green, Caroline A, Howarth, Jo, Partanen, R H, Pollock, Steven, Waterworth, Alison, Speirs, Valerie, Hughes, Thomas A, Hanby, Andrew M
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; beta Catenin - analysis; beta Catenin - biosynthesis; beta-catenin; Biological and medical sciences; Biomarkers, Tumor - analysis; Biomarkers, Tumor - metabolism; breast; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; cancer; Carcinoma - metabolism; Carcinoma - pathology; Cell Nucleus - metabolism; epithelial; Female; fibroblast; Fibroblasts - metabolism; Fibroblasts - pathology; Fluorescent Antibody Technique; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemistry; interaction; Investigative techniques, diagnostic techniques (general aspects); Mammary gland diseases; Medical sciences; Middle Aged; Neoplasm Grading; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Prognosis; stromal; Transfection; Tumor Microenvironment - physiology; Tumors
• ISSN: 0309-0167; 1365-2559
• DOI: 10.1111/j.1365-2559.2011.03917.x

Verghese E T, Shenoy H, Cookson V J, Green C A, Howarth J, Partanen R H, Pollock S, Waterworth A, Speirs V, Hughes T A & Hanby A M
(2011) Histopathology59, 609–618 Epithelial–mesenchymal interactions in breast cancer: evidence for a role of nuclear localized β‐catenin in carcinoma‐associated fibroblasts Aims:  Characteristics of the stroma around tumours are critical in defining the behaviour of cancers. β‐Catenin is well established as a critical regulator of carcinogenesis, acting as a transcriptional co‐activator in the nuclei of epithelial cancer cells. We have examined the prevalence and influence of nuclear β‐catenin within the stromal fibroblasts of breast cancer. Methods and results:  We examined β‐catenin expression in 201 breast cancers and adjacent normal tissue. Fibroblasts expressing nuclear β‐catenin were present in a significantly greater proportion of tumour tissues than normal tissues. The presence of fibroblasts with nuclear β‐catenin in tumours correlated with survival; tumours with prevalent positive fibroblasts were associated significantly with relatively good prognoses. Functional studies to examine influences of fibroblasts with nuclear β‐catenin, showed fibroblasts transfected to allow overexpression of β‐catenin were capable of inducing increases in both proliferation and invasion of breast cancer cell lines. Conclusion:  The presence of fibroblasts with nuclear β‐catenin in tumours is a good prognostic indicator, although in the context of tissue culture models these cells can increase the growth and metastatic potential of cancer cells. These apparently paradoxical observations underline the complexity of epithelial–stromal signalling within tumours and highlight an area for further study.