Calorie restriction (CR) reduces age-dependent decline of non-homologous end joining (NHEJ) activity in rat tissues

Even though CR has shown to enhance base excision repair (BER) and nucleotide excision repair (NER) capacities, it has not been reported whether CR can enhance non-homologous end joining (NHEJ) activity. To examine the effect of CR on NHEJ activity, ad libitum (AL)- and calorie restricted (CR)-diete...

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Published in:Experimental gerontology 2011-11, Vol.46 (11), p.891-896
Main Authors: Lee, Jae-Eun, Heo, Jee-In, Park, Seong-Hoon, Kim, Jeong-Hyeon, Kho, Yoon-Jung, Kang, Hong-Jun, Chung, Hae Young, Yoon, Jong-Lull, Lee, Jae-Yong
Format: Article
Language:English
Subjects:
Aging
Aging - genetics
Aging - metabolism
Animals
Blotting, Western
Caloric Restriction
Calorie restriction
DNA End-Joining Repair - genetics
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Kidney - metabolism
Liver - metabolism
Lung - metabolism
Male
NHEJ
Rats
Rats, Inbred F344
Reactive Oxygen Species
Spleen - metabolism
Up-Regulation
XRCC4
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Summary:Even though CR has shown to enhance base excision repair (BER) and nucleotide excision repair (NER) capacities, it has not been reported whether CR can enhance non-homologous end joining (NHEJ) activity. To examine the effect of CR on NHEJ activity, ad libitum (AL)- and calorie restricted (CR)-dieted rats were used. Age-dependent decline of NHEJ activity was apparent in the lung, liver, and kidney and appeared to be slightly decreased in spleen. CR reduced age-dependent decline of NHEJ activity in all tissues, even though the extent of recovery was variable among tissues. Moreover, CR appeared to reduce age-dependent decline of XRCC4 protein level. These results suggest that CR could reduce age-dependent decline of NHEJ activity in various tissues of rats possibly through up-regulation of XRCC4. ► CR reduced age-dependent decline of NHEJ activity in the lung, liver, kidney and spleen of rats. ► CR also appeared to reduce age-dependent decline of XRCC4 protein level. ► CR could reduce age-dependent decline of NHEJ activity in various tissues of rats possibly through up-regulation of XRCC4.
ISSN:0531-5565
1873-6815
DOI:10.1016/j.exger.2011.07.009