C-Reactive protein-directed immobilization of phosphocholine ligands on a solid surface

The complexes of C-reactive protein (CRP) with its polymerizable phosphocholine ligands adsorbed at the styrene-water interface were polymerized. The molecularly imprinted polymer (MIP) exhibited a binding affinity for CRP comparable to that of immobilized anti-CRP antibody. The determination of hum...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2011-11, Vol.47 (43), p.11900-11902
Main Authors: Kim, Eunjoo, Kim, Hyun-Chul, Lee, Se Geun, Lee, Sung Jun, Go, Tae-Jung, Baek, Chul Su, Jeong, Sang Won
Format: Article
Language:English
Subjects:
Antibodies, Immobilized - immunology
C-Reactive Protein - analysis
C-Reactive Protein - immunology
Humans
Immunoassay - methods
Ligands
Molecular Imprinting
Oils - chemistry
Phosphorylcholine - chemistry
Polystyrenes - chemistry
Surface Properties
Water - chemistry
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Summary:The complexes of C-reactive protein (CRP) with its polymerizable phosphocholine ligands adsorbed at the styrene-water interface were polymerized. The molecularly imprinted polymer (MIP) exhibited a binding affinity for CRP comparable to that of immobilized anti-CRP antibody. The determination of human serum CRP using the MIP-based sandwich immunoassay has been demonstrated.
ISSN:1359-7345
1364-548X
DOI:10.1039/c1cc15079k